β-catenin disruption decreases macrophage exosomal α-SNAP and impedes Treg differentiation in acute liver injury

Ruobin Zong; Yujie Liu; Mengya Zhang; Buwei Liu; Wei Zhang; Hankun Hu; Changyong Li

doi:10.1172/jci.insight.182515

β-catenin disruption decreases macrophage exosomal α-SNAP and impedes Treg differentiation in acute liver injury

Ruobin Zong,¹ Yujie Liu,¹ Mengya Zhang,¹ Buwei Liu,¹ Wei Zhang,¹ Hankun Hu,² and Changyong Li¹

Published November 19, 2024 - More info

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Abstract

Hepatic macrophages and regulatory T cells (Tregs) play an important role in the maintenance of liver immune homeostasis, but the mechanism by which hepatic macrophages regulate Tregs in acute liver injury remains largely unknown. Here, we found that the hepatic Treg proportion and β-catenin expression in hepatic macrophages were associated with acetaminophen (APAP) and D-galactosamine (D-GalN)/ lipopolysaccharide (LPS)-induced acute liver injury. Interestingly, β-catenin was markedly upregulated only in infiltrating macrophages, but not in resident Kupffer cells. Myeloid-specific β-catenin knockout mice showed an increased inflammatory cell infiltration and hepatocyte apoptosis. Moreover, myeloid β-catenin deficiency decreased the hepatic Treg proportion in the injured liver. Mechanistically, in vitro co-culture experiments revealed that macrophage β-catenin modulated its exosome composition, and influenced Treg differentiation. Using mass spectrometry-based proteomics, we identified that macrophage β-catenin activation increased the level of exosomal α-SNAP, which in turn promoted Treg differentiation. Overall, our findings demonstrated a molecular mechanism that macrophage β-catenin regulated the Treg proportion in the liver by enhancing the expression of exosomal α-SNAP, providing insights into the pathophysiology of acute liver injury.

β-catenin disruption decreases macrophage exosomal α-SNAP and impedes Treg differentiation in acute liver injury

Ruobin Zong,¹ Yujie Liu,¹ Mengya Zhang,¹ Buwei Liu,¹ Wei Zhang,¹ Hankun Hu,² and Changyong Li¹

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β-catenin disruption decreases macrophage exosomal α-SNAP and impedes Treg differentiation in acute liver injury

Ruobin Zong,1 Yujie Liu,1 Mengya Zhang,1 Buwei Liu,1 Wei Zhang,1 Hankun Hu,2 and Changyong Li1

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Ruobin Zong,¹ Yujie Liu,¹ Mengya Zhang,¹ Buwei Liu,¹ Wei Zhang,¹ Hankun Hu,² and Changyong Li¹