ResearchIn-Press PreviewImmunologyNephrology
Open Access | 10.1172/jci.insight.186892
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Huang, Z. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Dong, J. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Fu, Z. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Li, L. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by liu, S. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Wu, L. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Guo, H. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
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1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
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1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
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1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
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1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Crowley, S. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
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Ren, J.
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1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Kong, X. in: PubMed | Google Scholar
1Department of Nephrology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
2Department of Cardiology, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
3Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, United States of America
Find articles by Mao, H. in: PubMed | Google Scholar
Published July 29, 2025 - More info
Low-intensity pulsed ultrasound stimulation (LIPUS) has become increasingly appreciated for its therapeutic effect on kidney diseases. However, its role and biological mechanism in treating chronic kidney disease (CKD) remain poorly defined. Here, we revealed that LIPUS was applied in a safe range with an intensity of 25-315 mW/cm2. Daily LIPUS at an intensity of 315 mW/cm2 ameliorated ischemia-reperfusion (IR)-induced tubular injury and renal fibrosis, accompanied by the remarkable downregulation of IL-1R. Transcriptome sequencing showed that LIPUS significantly down-regulated IL-1R and its downstream genes in IL-1β-stimulated IR-injured mice. LIPUS effectively reversed IL-1β-induced tubular injury and reduced the production of profibrotic cytokines by down-regulating IL-1R in vivo and in vitro. Renal proximal tubule-specific Il1r1 knockout mice exhibited milder renal tubular injury and fibrosis after IR injury. However, LIPUS did not ameliorate IR injury in proximal tubule-specific Il1r1 knockout mice. Collectively, daily LIPUS at an intensity of 315 mW/cm2 relieves IR-induced tubular injury and fibrosis, potentially through down-regulating tubular IL-1R.