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Open Access | 10.1172/jci.insight.145228
1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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Sharma, C.
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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Singh, D.
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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1Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
2Signal Transduction Laboratory-1, National Institute of Immunology, New Delhi, India
3Department of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
4Department of Immunobiology, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
5Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
6Department of Immunology, International Centre for Genetic Engineering and Biotechnology, New Delhi, India
7Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
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Published January 14, 2021 - More info
Novel prime-boost immunization strategies are required to control the global Tuberculosis (TB) pandemic, which claims approximately 3 lives every minute. Here, we have generated an immunogenic complex against Mycobacterium tuberculosis (M.tb), consisting of promiscuous T cell epitopes (M.tb peptides) and TLR ligands assembled in liposomes. Interestingly, this complex (PTLs; peptide-TLR agonist-liposomes) induced significant activation of CD4+ T cells and IFNγ production in the PBMCs derived from PPD+ healthy individuals as compared to PPD- controls. Furthermore, intranasal delivery of PTLs significantly reduced the bacterial burden in the infected mice by inducing M.tb specific polyfunctional (IFNγ+IL17+TNFα+IL2+) immune responses and long-lasting central memory responses thereby reducing the risk of TB recurrence in DOTS treated infected animals. The transcriptome analysis of peptide-stimulated immune cells unveiled the molecular basis of enhanced protection. Furthermore, PTLs immunization significantly boosted the BCG-primed immune responses against TB. The greatly enhanced efficacy of BCG-PTLs vaccine model in controlling pulmonary TB projects PTLs as an adjunct vaccine against TB.