Identification and characterization of a supraclavicular brown adipose tissue in mice
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
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Research Article Development Endocrinology

Identification and characterization of a supraclavicular brown adipose tissue in mice

Abstract

A fundamental challenge to our understanding of brown adipose tissue (BAT) is the lack of an animal model that faithfully represents human BAT. Such a model is essential for direct assessment of the function and therapeutic potential of BAT depots in humans. In human adults, most of the thermoactive BAT depots are located in the supraclavicular region of the neck, while mouse studies focus on depots located in the interscapular region of the torso. We recently discovered BAT depots that are located in a region analogous to that of human supraclavicular BAT (scBAT). Here, we report that the mouse scBAT depot has morphological characteristics of classical BAT, possesses the potential for high thermogenic activity, and expresses a gene signature that is similar to that of human scBAT. Taken together, our studies reveal a mouse BAT depot that represents human BAT and provides a unique tool for developing new translatable approaches for utilizing human scBAT.

Authors

Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen

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Figure 7

Validation of expression of novel and known genes in mouse scBAT.

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Validation of expression of novel and known genes in mouse scBAT. (A) Va...
(A) Validation of RNA-sequencing results by qRT-PCR. Heat map of expression levels of 10 randomly selected genes from the 61 genes in Figure 6E in iBAT, scBAT, iWAT, and eWAT from 8-week-old mice. The heat map was constructed using the log2-transformed mean value of each tissue normalized to the median value of all samples in all 4 different adipose tissues. n = 5. (B) Relative mRNA expression of Lhx1, Bglap2, and Cst10 (Cst10 was absent in iBAT) in iBAT, scBAT, iWAT, and eWAT isolated from 8-week-old mice. Data are presented as mean ± SEM. n = 5. ****P < 0.0001, ***P < 0.001, *P < 0.05. One-way ANOVA. (C) Relative mRNA expression of Lhx1 and Bglap2 in differentiated P7scBAT-C4 cells. Data are presented as mean ± SEM. n = 3. **P < 0.01, *P < 0.05. Two-tailed t test. CON, treated with vehicle (ethanol [EtOH]); IM, induction medium. (D) Relative mRNA expression of Kcnk3 and Mtus1 in iBAT, scBAT, iWAT, and eWAT. Data are presented as mean ± SEM. n = 5. **P < 0.01, *P <0.05. One-way ANOVA. (E) Relative mRNA expression of Kcnk3 and Mtus1 in P7scBAT-C4 cells. Data are presented as mean ± SEM. n = 3. **P < 0.01, *P <0.05. Two-tailed t test.