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Resource and Technical AdvanceIn-Press PreviewImmunologyOncology Open Access | 10.1172/jci.insight.190703

Living human lung slices for ex vivo modelling of lung cancer

Siavash Mansouri,1 Annika Karger,1 Clemens Ruppert,2 Marc A. Schneider,3 Andreas Weigert,4 Rajender Nandigama,1 Blerina Aliraj,4 Lisa Strotmann,3 Anoop V. Cherian,1 Diethard Pruefer,5 Peter Dorfmuller,1 Ludger Fink,6 Ibrahim Alkoudmani,7 Stefan Gattenlöhner,8 Bastian Eul,2 Andre Althoff,9 Peter Kleine,10 Hauke Winter,11 Andreas Guenther,1 Ardeschir Ghofrani,1 Soni S. Pullamsetti,1 Friedrich Grimminger,1 Werner Seeger,1 and Rajkumar Savai1

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Mansouri, S. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Karger, A. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Ruppert, C. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Schneider, M. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Weigert, A. in: PubMed | Google Scholar |

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Nandigama, R. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Aliraj, B. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Strotmann, L. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Cherian, A. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Pruefer, D. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Dorfmuller, P. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Fink, L. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Alkoudmani, I. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Gattenlöhner, S. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Eul, B. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Althoff, A. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Kleine, P. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Winter, H. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Guenther, A. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Ghofrani, A. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Pullamsetti, S. in: PubMed | Google Scholar |

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Grimminger, F. in: PubMed | Google Scholar

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Seeger, W. in: PubMed | Google Scholar |

1Institute for Lung Health, Justus Liebig University, Giessen, Germany

2Department of Internal Medicine, Member of the DZL, Member of CPI, Justus Liebig University, Giessen, Germany

3Translational Research Unit, Thoraxklinik at Heidelberg University Center, Heidelberg, Germany

4Institute of Biochemistry I, Faculty of Medicine, Goethe University Frankfurt, Frankfurt, Germany

5Department of Thoracic Surgery, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany

6Institute of Pathology, Dermatopathology, Cytology and Molecular Pathology, UEGP, Wetzlar, Germany

7Department of General and Thoracic Surgery, University Hospital Giessen, Giessen, Germany

8Department of Pathology, Justus Liebig University, Giessen, Germany

9Department of Pulmonology, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

10Department of Thoracic Surgery, Thoraxzentrum Offenbach, Sana Klinikum Offenbach, Offenbach, Germany

11Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany

Find articles by Savai, R. in: PubMed | Google Scholar |

Published July 29, 2025 - More info

JCI Insight. https://doi.org/10.1172/jci.insight.190703.
Copyright © 2025, Mansouri et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published July 29, 2025 - Version history
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Abstract

The tumor microenvironment (TME) significantly impacts cancer progression, yet traditional animal models do not fully recapitulate the situation in humans. To address this, we developed tumor-derived precision lung slices (TD-PCLS), an ex vivo platform for studying the lung TME and evaluating therapies. TD-PCLS, viable for 8 to 10 days, preserve the heterogeneity and metabolic activity of primary tumors, as confirmed by seahorse analysis. Using multispectral FACS and phenocycler multiplex imaging, we spatially profiled TME components and cancer cell functionality. Additionally, TD-PCLS revealed patient-specific responses to chemo- and immunotherapies. To complement TD-PCLS, we established tumor-cell-seeded PCLS (TCS-PCLS) by introducing tumor and immune cells into healthy lung slices. This model highlighted macrophage-tumor interactions as critical for tumor cell proliferation, migration, and immune modulation. Together, these platforms provide a robust tool for lung cancer research, enabling precision medicine and advancing therapeutic discovery.

Graphical Abstract
graphical abstract
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View Supplemental Video 1. Time-lapse imagings of GFP-positive A549 cells on healthy PCLS.

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  • Version 1 (July 29, 2025): In-Press Preview
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