Research ArticleNephrology
Open Access | 10.1172/jci.insight.163306
1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
Find articles by Hayashi, T. in: JCI | PubMed | Google Scholar
1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
Find articles by Ojima, M. in: JCI | PubMed | Google Scholar
1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
Find articles by Ishibashi, S. in: JCI | PubMed | Google Scholar
1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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Kuno, A.
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1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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1Department of Anatomy and Embryology, Faculty of Medicine;
2PhD Program in Human Biology, School of Integrative and Global Majors;
3Department of Nephrology, Faculty of Medicine; and
4Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
5Tohoku Medical Megabank Organization and
6Advanced Research Center for Innovations in Next-Generation Medicine (INGEM), Tohoku University, Sendai, Japan.
7Laboratory Animal Resource Center,
8Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),
9International Institute for Integrative Sleep Medicine (WPI-IIIS), and
10Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
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Published February 14, 2023 - More info
The transcription factor c-Maf has been widely studied and has been reported to play a critical role in embryonic kidney development; however, the postnatal functions of c-Maf in adult kidneys remain unknown as c-Maf–null C57BL/6J mice exhibit embryonic lethality. In this study, we investigated the role of c-Maf in adult mouse kidneys by comparing the phenotypes of tamoxifen-inducible (TAM-inducible) c-Maf–knockout mice (c-Maffl/fl; CAG-Cre-ERTM mice named “c-MafΔTAM”) with those of c-Maffl/fl control mice, 10 days after TAM injection [TAM(10d)]. In addition, we examined the effects of c-Maf deletion on diabetic conditions by injecting the mice with streptozotocin, 4 weeks before TAM injection. c-MafΔTAM mice displayed primary glycosuria caused by sodium-glucose cotransporter 2 (Sglt2) and glucose transporter 2 (Glut2) downregulation in the kidneys without diabetes, as well as morphological changes and life-threatening injuries in the kidneys on TAM(10d). Under diabetic conditions, c-Maf deletion promoted recovery from hyperglycemia and suppressed albuminuria and diabetic nephropathy by causing similar effects as did Sglt2 knockout and SGLT2 inhibitors. In addition to demonstrating the potentially unique gene regulation of c-Maf, these findings highlight the renoprotective effects of c-Maf deficiency under diabetic conditions and suggest that c-Maf could be a novel therapeutic target gene for treating diabetic nephropathy.
c-Maf is a member of the large Maf family of basic leucine zipper transcription factors. The leucine zipper domain mediates dimer formation, and its basic region binds to the Maf recognition element (MARE) in genomic DNA. There are 3 types of MAREs, namely TRE-type, CRE-type, and half-MARE (1). In addition to these domains, large Maf family members are characterized by the presence of an N-terminal acidic transactivation domain (2). The functions of c-Maf have been widely studied, and it has been reported to play critical roles in the embryonic stage, including in the differentiation of T lymphocyte subsets (3, 4), differentiation involving γ-crystallin synthesis in lens fiber cells (5, 6), differentiation of hypertrophic chondrocytes during endochondral bone development (7, 8), and development of the embryonic kidney and liver cells. In mice, c-Maf is first expressed on embryonic day 16 in the proximal tubules (9); however, the postnatal functions of c-Maf in adult kidneys remain unclear as c-Maf–null mice in a C57BL/6J background exhibit embryonic lethality (10). Therefore, to investigate the functions of c-Maf in adult kidneys, we previously generated tamoxifen-inducible (TAM-inducible) c-Maf–knockout (c-Maffl/fl; CAG-Cre-ERTM) mice, which we named “c-MafΔTAM” mice (11).
Transporters mediate the translocation of substrates across biological membranes and are widely expressed throughout the body, including the liver, brain, and kidney. There are 2 main transporter superfamilies, namely the ATP-binding cassette (ABC) superfamily and the solute carrier (SLC) superfamily. ABC transporters use energy from ATP hydrolysis and function as efflux transporters, whereas SLC transporters are associated with the cellular uptake of small molecules. Transporters from both families have attracted considerable attention in drug development as over 80 SLC transporters are known to be involved in monogenic disorders (12) and, thus, have therapeutic potential.
The kidneys reabsorb glucose through specific transporters, including sodium-glucose cotransporters (SGLTs) and glucose transporters (GLUTs) (13). SGLT2 is considered a major glucose reabsorption transporter as the reabsorption ratio of SGLT2-filtered glucose is 80%–90% in the proximal tubules (14, 15). Importantly, the pharmacological inhibition of SGLT2 reduces hyperglycemia and glomerular hyperfiltration by suppressing renal glucose reabsorption to increase the excretion of urinary glucose, thereby reducing the risk of progression to chronic kidney disease (CKD) (16–19). SGLT2 inhibitors have, therefore, received increasing attention as a target for a new class of blood glucose–lowering drugs that have been approved to treat type 2 diabetes mellitus and CKD.
In addition, it was recently reported that induction of renal Glut2 deficiency reversed hyperglycemia in streptozotocin-induced (STZ-induced) mouse models (20). Glut2 is localized in renal proximal tubule cells and normally affects the basolateral efflux of the reabsorbed glucose from the tubular cells back into the circulation. Hyperglycemia induces Glut2 expression in the kidney, and its increased expression contributes to the development of diabetic kidney disease (DKD) in mice. Renal proximal tubule cell–specific ablation of Glut2 has been demonstrated to ameliorate the progress of DKD in mice because of suppression of cannabinoid-1 receptor stimulation and mTOR complex 1 (mTORC1) activity (21, 22). Therefore, in addition to Sglt2, Glut2 can be established as a target gene for the treatment of diabetes. However, the physiological contributions of Sglt2 and Glut2 in the kidney to systemic glucose homeostasis have been not completely established; therefore, it is important to elucidate the mechanisms underlying the function of these transporters.
This study was aimed at investigating the role of c-Maf in adult mice by exploring the specific phenotypes of c-MafΔTAM mice and characterizing the associated c-Maf transcriptional profiles using RNA sequencing (RNA-Seq) and quantitative polymerase chain reaction (qPCR). In addition, we evaluated the direct binding of c-Maf with Sglt2 and Glut2 and explored the effects of c-Maf deletion on diabetes and kidney injury. Overall, in this study, we found potentially novel aspects of c-Maf regulation in the kidneys and provided causal insights into c-Maf regulation and the phenotypes of c-MafΔTAM mice, including urinary features and improvements in diabetic conditions and nephropathy progression.
c-MafΔTAM mice develop severe renal glycosuria, basic aminoaciduria, and albuminuria. To explore the roles of c-Maf in adults, we analyzed the urine and blood components of 8-week-old c-MafΔTAM and c-Maffl/fl (control) mice, 10 days after TAM injection [TAM(10d)]. First, we found that urinary glucose levels were sharply increased in c-MafΔTAM mice compared with those in c-Maffl/fl mice on TAM(10d) (Figure 1A). In addition, water intake and urine volume were higher in c-MafΔTAM mice than in the control mice (Figure 1, B and C). Together, these 3 conditions are typical signs of diabetes; however, blood glucose levels in c-MafΔTAM mice were only slightly lower than in the control mice (Figure 1D). In addition, intraperitoneal glucose tolerance tests (IPGTTs), serum insulin and glucagon levels, and insulin receptor substrate 1 (IRS1) and IRS2 mRNA expression levels were comparable between the 2 groups (Figure 1, E–G, and Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.163306DS1). Moreover, to examine c-Maf expression, morphological changes upon c-Maf deletion, and c-Maf function in the pancreas, IHC staining, hematoxylin and eosin (HE) staining, and analysis of serum amylase, whose value changes when there is an abnormality in the pancreas, were performed. The IHC staining revealed c-Maf expression around the exocrine cells, such as the macrophages and interstitial area, but not in the islet cells (Supplemental Figure 1C). Although its expression in c-MafΔTAM mice decreased compared with that in c-Maffl/fl mice, there were no morphological changes and no significant difference in amylase levels in the serum under the feeding conditions compared with those in c-Maffl/fl mice (Supplemental Figure 1, D and E).
c-MafΔTAM mice develop severe renal glycosuria, basic aminoaciduria, and albuminuria. Changes in (A) urine glucose, (B) water intake, and (C) urine volume in c-MafΔTAM and c-Maffl/fl groups (n = 7 per group). c-MafΔTAM group showed higher urinary glucose level, water intake, and urine volume. Loss of c-Maf did not alter glucose homeostasis, as determined by (D) 4-hour fasting blood glucose level, (E) glucose tolerance test after 10 hours of fasting, (F) serum insulin level, and (G) serum glucagon level. Glucose tolerance was examined before and up to 120 minutes after glucose solution injection (n = 11 in c-Maffl/fl and n = 15 in c-MafΔTAM groups). (H) Urinary basic amino acid levels increased in the c-MafΔTAM mice compared with the c-Maffl/fl mice. (I) c-MafΔTAM group showed higher urinary albumin levels than the c-Maffl/fl group (n = 6 per group). (J) IHC staining for albumin expression in the c-MafΔTAM group showed decreased granular signals in the proximal tubules compared with the c-Maffl/fl group (n = 3 per group). A analyses were performed on TAM(10d). Scale bars: 100 μm. A–I were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. *P < 0.05, and ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
In addition, we examined the liver and small intestine, along with the pancreas, because the roles of c-Maf in adult liver and small intestine were recently reported (23, 24). In the case of liver, c-Maf was expressed around the hepatic cells, such as sinusoidal cells and macrophages (Supplemental Figure 1F). Although there was a tendency for decreased c-Maf expression, no morphological changes were observed (Supplemental Figure 1G). In addition, there were no significant differences in serum aspartate transaminase and alanine transaminase levels, which are criteria for liver function (Supplemental Figure 1, H and I). In fact, time-specific c-Maf loss did not alter the fractal dimension patterning and did not induce liver damage, such as fibrosis, under basal conditions (23). Considering our data and the results reported previously, we concluded that c-Maf deletion does not have primary and critical effects on the function and morphology of the liver in this study.
In the case of small intestine, there was a tendency for decreased c-Maf expression (Supplemental Figure 1J); however, c-MafΔTAM mice did not show morphological changes in the small intestine compared with that in c-Maffl/fl mice (Supplemental Figure 1K). In fact, although in enterocytes, the expression of c-Maf plays an important role in nutrient absorption, TAM-induced c-Maf deletion in the entire intestinal epithelium did show comparable change in the body weight and unaltered nutrient absorption capacity with no compensatory increase in other MAF family members (24). Considering our data and the result reported previously, we concluded that c-Maf deletion does not have primary and critical effects on the function and morphology of the small intestine as well as of the pancreas and liver in this study. Taken together, we concluded that the significant increase in urinary glucose levels was due to primary glycosuria, not diabetes.
In addition to primary glycosuria, the c-MafΔTAM mice showed a tendency for increased urinary basic amino acid levels compared with c-Maffl/fl mice on TAM(10d) (Figure 1H); however, the total serum concentrations of these amino acids did not differ significantly (Supplemental Figure 1L). High urinary basic amino acid levels are a typical feature of Fanconi renotubular syndrome (FRTS; Online Mendelian Inheritance in Man [OMIM]: 134600, 613388, 615605, 616026, 618913) (25). Consistently, the mice displayed some other characteristics of FRTS, including increased renal sodium and a tendency for increased urinary phosphate levels, potassium, and calcium wasting; a tendency for lower urinary uric acid levels; and no significant differences in serum sodium and potassium levels (Supplemental Figure 1, M–S), indicating that FRTS was incomplete in c-MafΔTAM mice.
We also found that urinary albumin levels were higher on TAM(10d) in c-MafΔTAM mice than in c-Maffl/fl mice because of reduced albumin reabsorption in the proximal tubules, as indicated by immunohistochemistry (IHC) staining (Figure 1, I and J). Since high urinary albumin levels are a typical marker of nephropathy, we performed histological and biochemical analysis on the kidneys. Physiological serum creatinine and urea nitrogen (UN) levels did not differ significantly between the MafΔTAM and c-Maffl/fl mice (Supplemental Figure 1, T and U). Furthermore, histological analysis using HE and periodic acid–Schiff staining as well as electron microscopy images revealed no significant structural changes in the glomeruli or brush border membranes of the proximal tubules in c-MafΔTAM and c-Maffl/fl mice on TAM(10d) (Supplemental Figure 1V).
Given that c-MafΔTAM mice can survive for at least 2 years following TAM injection (n = 6, data not shown), it is possible that c-Maf loss leads to renal glycosuria, basic aminoaciduria and albuminuria without diabetes, complete FRTS, morphological changes, and life-threatening kidney injuries.
Relationship between c-Maf and renal features in c-MafΔTAM mice. To determine why glycosuria, aminoaciduria, and albuminuria occurred in c-MafΔTAM mice from the perspective of genetic variation, we performed RNA-Seq and qPCR analyses in both the groups. A total of 374 differentially expressed genes (DEGs) were detected between c-MafΔTAM and c-Maffl/fl mice (Figure 2A), among which 261 were upregulated and 113 were downregulated. Pathway analysis of these DEGs indicated a strong relationship between c-Maf and SLC-mediated transmembrane transport (Figure 2B). To obtain further insights into the regulation of c-Maf in specific clusters, we constructed a k-means clustering heatmap (Figure 2C) and selected 1 cluster with clear differences in both groups (Supplemental Figure 2A). This cluster included 2 glucose transporters, Slc5a2 (Sglt2) and Slc2a2 (Glut2), through which renally filtered glucose is reabsorbed in the proximal tubules (13, 26), suggesting that primary glycosuria in c-MafΔTAM mice is caused by decreased expression of these transporters on TAM(10d). In addition, the cluster included several amino acid transporters that were downregulated in c-MafΔTAM mice, which may result in aminoaciduria (Supplemental Figure 2B). In terms of albuminuria, qPCR analysis suggested that the gene expression of megalin (Lrp2) and cubilin (Cubn), which are responsible for albumin reabsorption in proximal tubules, was downregulated in c-MafΔTAM mice (Figure 2, D and E). In addition, IHC staining revealed a tendency for decreased megalin expression in proximal tubules of c-MafΔTAM mice compared with that in c-Maffl/fl mice; no negative feedback was observed at least at the mRNA level (Figure 2F). Indeed, previous studies using Lrp2- and Cubn-deficient mice have reported no significant morphological changes in the kidneys due to deficiency of these 2 genes (27, 28). Together, these results elucidate c-Maf gene regulation in kidney proximal tubules and demonstrate that c-Maf deletion leads to renal glycosuria, basic aminoaciduria, and albuminuria by downregulating the gene expression of several transporters in the kidney.
RNA-Seq analysis of the relationship between c-Maf and renal features. (A) Volcano plot of overall gene expression: 261 genes were upregulated (red) and 113 genes were downregulated (blue) in c-MafΔTAM mice. (B) Top 10 pathways of the DEGs in c-MafΔTAM mice. (C) Heatmap of the expression of 123 genes related to SLC-mediated transmembrane transport between c-MafΔTAM and c-Maffl/fl mice (n = 4 per group). (D) Megalin and (E) cubilin gene expression levels were lower in c-MafΔTAM mice than in c-Maffl/fl mice (n = 10 per group). (F) A tendency for decreased megalin protein expression levels in c-MafΔTAM mice than in c-Maffl/fl mice (n = 4 per group). Scale bars: 100 μm. For RNA-Seq transcriptome analysis, gene expression values were calculated using the quantile method. Gene expression was compared between c-Maffl/fl control mice and c-MafΔTAM mice using the Empirical Analysis DGE tool (edgeR test) in the CLC Genomics Workbench (Version 10.1.1; QIAGEN). False discovery rate (FDR) adjustments (81) were applied to correct for multiple testing, and DEGs were identified using an FDR cutoff of 0.05. D and E were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. *P < 0.05, and ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
c-Maf is a potentially novel transcription factor that regulates Sglt2 and Glut2. To verify the direct regulation of Sglt2 and Glut2 by c-Maf, we evaluated the RNA expression levels of these transporters using qPCR. The expression of Sglt2 and Glut2 was significantly lower in the kidneys of c-MafΔTAM mice, consistent with the observed reduction in c-Maf expression (Figure 3, A and B, and Supplemental Figure 3A). Moreover, Glut1 expression was unchanged and Sglt1 expression was slightly increased, likely to compensate for Sglt2 downregulation (Supplemental Figure 3, B and C) (29). IHC analysis further verified that the expression of Sglt2 and Glut2 proteins was substantially decreased in c-MafΔTAM mice compared with that in the c-Maffl/fl control mice (Figure 3, C and D). Moreover, localization of the 2 transporters was consistent with that of c-Maf in the proximal tubules of the kidneys in c-MafLacZ/+ mice (Figure 3, E and F). Next, we examined c-Maf, Sglt2, Glut2, and hepatocyte nuclear factor 1α (HNF1α) in the kidneys at 1 day after the second TAM injection [TAM(1d)]. c-Maf and Sglt2 expression in c-MafΔTAM mice was significantly reduced, and there was tendency for decreased Glut2 expression even 1 day after the second injection compared with that in the c-Maffl/fl mice (Figure 3, G–I); in contrast, there was no significant difference in HNF1α expression in both groups on TAM(10d) (Figure 3J). In fact, in the previous study, it was concluded that renal Glut2 deficiency reduced the expression of Sglt2 on day 14 after the third TAM injection via downregulation of the transcription factor HNF1α (20). However, in this study, c-Maf deletion significantly decreased the expression of Sglt2 on only a day after the second TAM injection, suggesting that the time lag in the reduction of Sglt2 expression between previous studies and this study indicates direct regulation of Sglt2 by c-Maf. Therefore, these results suggest that c-Maf directly controls the expression of Sglt2 and Glut2 in the kidney.
c-Maf ΔTAM mice show decreased Sglt2 and Glut2 gene and protein expression. (A) Sglt2 and (B) Glut2 mRNA levels were analyzed in kidney tissues from c-Maffl/fl and c-MafΔTAM mice using qPCR (n = 12 per group) on TAM(10d). (C) Sglt2 and (D) Glut2 protein expression was decreased in the kidney cortex of c-MafΔTAM mice compared with c-Maffl/fl mice (n = 4 per group). (E and F) c-Maf colocalization with Sglt2 and Glut2 in consecutive sections (n = 3). (G) c-Maf, (H) Sglt2, (I) Glut2, and (J) HNF1α mRNA level was analyzed in kidney tissues from c-Maffl/fl and c-MafΔTAM mice using qPCR (n = 3 per group) at 1 day after the second TAM injection. Blue numbers indicate the colocalization of c-Maf with the transporters Sglt2 and Glut2. For IHC analyses, 3-week-old c-MafΔTAM and c-Maffl/fl mice were injected with TAM to verify Sglt2 and Glut2 protein expression. The colocalization of the transporters with c-Maf was verified using 6-week-old c-MafLacZ/+ mice (n = 3 per group). A, B, and G–J were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. Scale bars: 100 μm. **P < 0.01, and ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
c-Maf directly controls the expression of Sglt2 and Glut2. Since the sequences upstream of the Sglt2 and Glut2 transcription start sites contain a TRE-type MARE and half-MARE, respectively, we performed chromatin immunoprecipitation (ChIP) and luciferase assays to verify that c-Maf controls Sglt2 and Glut2 by directly binding to their MARE sites (Figure 4, A, C, E, and G). The primer pairs constructed for these assays, including those for MAREs, are shown in Supplemental Tables 1 and 2. The ChIP assays revealed that the gene regulatory region amplification signals for Sglt2 and Glut2 were significantly reduced in the kidneys of c-MafΔTAM mice on TAM(10d) compared with that in c-Maffl/fl mice, suggesting that c-Maf bound to the MAREs in the Sglt2 and Glut2 gene regulatory regions (Figure 4, B and F). Luciferase assays confirmed that c-Maf significantly increased the activities of Sglt2 and Glut2 in the absence of MARE mutations but that their activities showed significantly lower responsiveness toward c-Maf in the presence of MARE mutations (Figure 4, D and H). Therefore, these results verify that c-Maf directly regulates Sglt2 and Glut2 and is associated with their expression in adult mouse kidneys, resulting in severe glycosuria.
c-Maf as a potentially novel Sglt2- and Glut2-regulating transcription factor. ChIP assay and luciferase reporter assay revealed the direct regulation of Sglt2 and Glut2 by c-Maf. Quantitative ChIP confirmed direct binding of c-Maf to MARE sites in (A and B) Sglt2 and (E and F) Glut2 loci; ChIP assay was performed upstream of the transcription start sites (primer pairs 1 for Sglt2 and 3 for Glut2), and intron sites as an additional negative control (primer pairs 2 for Sglt2 and 4 for Glut2). The luciferase reporter system examined the activation of (C and D) Sglt2 and (G and H) Glut2. Dual-luciferase reporter plasmids with or without a mutation in Sglt2 and Glut2 were cotransfected with the vectors carrying c-Maf (0–90 ng). Experiments were repeated 3 times. B and F were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. Holm-Šídák corrections were applied for multiple statistical tests in D and H. *P < 0.05, **P < 0.01, and ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
Improvements in diabetic conditions and kidney function in c-MafΔTAM mice. The clinical hallmarks of diabetes mellitus and diabetic nephropathy with CKD progression include hyperglycemia, a high glomerular filtration rate, and increased urinary albumin excretion (30–34). It has been reported that STZ-treated mice exhibit hyperglycemia, hyperfiltration, and high urinary albumin levels compared with untreated mice and that these aspects are improved in Sglt2-knockout mice (35) and by SGLT2 inhibitors (36–38). Therefore, we examined whether c-Maf deletion had similar effects on diabetes, blood glucose levels, creatinine clearance rate (Ccr), osmolar clearance, serum creatinine and UN levels, and urinary albumin levels in STZ-induced diabetic c-MafΔTAM mice. Details of the experimental protocol are provided in Supplemental Figure 4A. Although we were unable to distinguish differences in c-Maf localization in the proximal tubules, c-Maf gene expression was significantly lower in the kidneys of c-Maffl/fl mice after STZ(7w) TAM(3w) than after TAM(8w) without STZ (Supplemental Figure 4B), and c-Maf protein expression was significantly decreased in the kidneys of c-MafΔTAM mice after STZ(12w) TAM(8w) compared with that in c-Maffl/fl mice (Supplemental Figure 4, C and D), likely due to proximal tubular damage. In addition, Sglt2 and Glut2 gene expression was significantly decreased in the kidneys of c-Maffl/fl mice after STZ(7w) TAM(3w) versus after TAM(8w) without STZ (Supplemental Figure 4, E and F), probably due to the decreasing c-Maf level. While there were no significant differences in body weight on STZ(4w) TAM(0w) between the groups, we observed a tendency for increased body weight in c-MafΔTAM mice on STZ(12w) TAM(8w) compared with that in the control mice, with no significant difference in food intake (Supplemental Figure 4, G and H).
As shown in Figure 5A, blood glucose levels in c-MafΔTAM mice were significantly reduced, 9 weeks after STZ and 5 weeks after TAM injection [STZ(9w) TAM(5w)], compared with those in c-MafΔTAM mice, 3 weeks after STZ and 1 week before TAM injection [STZ(3w) TAM(–1w)], despite the gradual progression of hyperglycemia in c-Maffl/fl mice. In fact, c-MafΔTAM mice showed higher urinary glucose levels than the control mice on STZ(12w) TAM(8w) (Supplemental Figure 4I). Although glucose intolerance was not attenuated in c-MafΔTAM mice on STZ(9w) TAM(5w) because of STZ-induced diabetes, similar to that in c-Maffl/fl mice (Figure 5B), Ccr, osmolar clearance, and blood pressure were decreased in c-MafΔTAM mice on STZ(12w) TAM(8w), suggesting that the suppression of glomerular hyperfiltration may decrease Ccr, osmolar clearance, and blood pressure, resulting in decreased urine volume (Figure 5, C–E, and Supplemental Figure 4J) (35). While serum creatinine levels did not change between c-MafΔTAM and c-Maffl/fl mice, serum UN levels improved (Figure 5, F and G).
c-Maf loss attenuates diabetic conditions. (A) c-MafΔTAM mice (n = 11) displayed significantly lower blood glucose levels after 10-hour fasting than c-Maffl/fl mice (B) but no significant difference in glucose intolerance between 2 groups. n = 6 on STZ(9w) TAM(5w). (C) Creatinine clearance rate, (D) osmolar clearance, and (E) urine volume were reduced in c-MafΔTAM mice, as a result of improved glomerular hyperfiltration, compared with control mice. [C: n = 5 per group on TAM(10d); c-Maffl/fl, n = 5 and c-MafΔTAM, n = 7 on STZ(12w). D: n = 5 per group on TAM(10d); c-Maffl/fl, n = 4 and c-MafΔTAM, n = 7 on STZ(12w). E: n = 6 per group on STZ(3w) TAM(-1w); c-Maffl/fl, n = 5 and c-MafΔTAM, n = 7 on STZ(12w) TAM(8w)]. Different mice were assessed on TAM(10d) and STZ(12w) TAM(8w). c-Maf deletion attenuated diabetic kidney nephropathy, as indicated by an improvement in (F) serum creatinine and (G) serum UN levels in c-MafΔTAM mice compared with c-Maffl/fl mice, as well as (H) urinary albumin levels on TAM(10d) (left) and STZ(12w) TAM(8w) (center) and glomerular urinary albumin levels on STZ(12w) TAM(8w) (right). [F and G: n = 5 per group on TAM(10d); c-Maffl/fl, n = 5 and c-MafΔTAM, n = 6 on STZ(12w). H: n = 8 per group on TAM(10d); c-Maffl/fl, n = 5 and c-MafΔTAM, n = 6 on STZ(12w)]. B–H were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. Holm-Šídák corrections were applied for multiple statistical tests in A. *P < 0.05, **P < 0.01, ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
Next, we examined glomerular urinary albumin levels, which indicate the total amount of albumin leaked from the glomerulus and are a typical marker of diabetic nephropathy. No significant differences were observed in urinary albumin levels, which represent the total amount of nephron albumin on STZ(12w) TAM(8w) between the 2 groups (Figure 5H); however, urinary albumin levels were higher in c-MafΔTAM mice on TAM(10d) than in c-Maffl/fl mice because of the downregulation of Lrp2 and Cubn genes, resulting in reduced albumin reabsorption in proximal tubules (Figure 5H and Figure 2D). Using a previously reported method (39) and the difference between total albumin levels on STZ(12w) TAM(8w) and urinary albumin levels on TAM(10d) (Figure 5H), we found that glomerular urine albumin levels were significantly reduced in c-MafΔTAM mice on STZ(12w) TAM(8w) compared with those in c-Maffl/fl mice (Figure 5H). This decrease in glomerular urinary albumin levels in c-MafΔTAM mice on STZ(12w) TAM(8w) could indicate improvements in diabetic nephropathy. In addition, these results demonstrated similar attenuations in hyperglycemia and hyperfiltration compared with those in Sglt2-knockout mice and in those treated with SGLT2 inhibitors and STZ injection, as well as lowered glomerular albuminuria in c-MafΔTAM mice. Therefore, these findings suggest that c-Maf loss exerts similar effects on hyperglycemia, other diabetic conditions, and kidney nephropathy as Sglt2 knockout and SGLT2 inhibitors.
Improvement of diabetic nephropathy in c-MafΔTAM mice. The observed decrease in glomerular albuminuria in c-MafΔTAM mice (Figure 5) suggests that the loss of c-Maf may improve nephropathy and diabetic status. Interestingly, previous studies using diabetic Sglt2-knockout mice have shown that the loss of Sglt2 can attenuate diabetic conditions, such as hyperglycemia and glomerular hyperfiltration, but not nephropathy (35). In contrast, diabetic mice treated with SGLT2 inhibitors reportedly showed improvement in the progression of diabetic nephropathy (40–43). Therefore, we investigated whether c-Maf deletion improves diabetic nephropathy by measuring periodic acid–methenamine silver–positive (PAMS-positive)and Sirius red–positive areas to examine the mesangial matrix index and fibrosis in the kidneys on STZ(12w) TAM(8w). PAMS staining was improved in c-MafΔTAM mice compared with that in control mice (Figure 6, A and B, and Supplemental Figure 5, A and B), and Sirius red–positive areas were significantly decreased (Figure 6, C and D, and Supplemental Figure 5, C and D). In addition, the expression of Col1a1, Col4a3, and fibronectin genes was reduced in c-MafΔTAM mice (Figure 6, E–G).
Diabetic kidney injury improves in c-MafΔTAM mice through reduced oxidative stress and inflammatory cytokine levels. Decreased (A and B) PAMS-positive mesangial matrix index and (C and D) Sirius red–positive area indicate the suppression of diabetic kidney injury in c-MafΔTAM mice (A and B: c-Maffl/fl, n = 5; c-MafΔTAM, n = 7. C and D: n = 5 per group). Kidney sections collected on STZ(12w) TAM(8w) were used for both staining procedures. The increased ratio of mesangial matrix and fibrogenesis in c-MafΔTAM mice was ameliorated compared with c-Maffl/fl mice. The mesangial matrix index represents the ratio of the mesangial matrix area (green) to the tuft area (glomerulus in red). Tubulointerstitial fibrogenesis lesions, defined as Sirius red–positive areas (blue), were measured to detect fibrogenesis and expressed as a percentage of the total area. (E) Col1a1, (F) Col4a3, and (G) fibronectin expression on STZ(12w) TAM(8w) (c-Maffl/fl, n = 5; c-MafΔTAM, n = 7). (H) IHC staining of 8-OHdG distribution in the kidneys STZ(12w) TAM(8w) (n = 4 per group). (I) Urinary 8-OHdG levels indicate reduced oxidative stress in the kidneys of c-MafΔTAM mice compared with c-Maffl/fl mice [n = 5 per group on TAM(10d); c-Maffl/fl, n = 5 and c-MafΔTAM, n = 7 on STZ(12w) TAM(8w)]. (J) Tgfβ and (K) Il-6 expression on STZ(12w) TAM(8w) (c-Maffl/fl, n = 5; c-MafΔTAM, n = 7). Scale bars: 100 μm. B, D–G, and I–K were presented as the mean and the standard error of the mean (SEM). To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P value < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001. White circles: c-Maffl/fl groups, and black circles: c-MafΔTAM groups.
Since oxidative stress is a key molecular mechanism underlying diabetic nephropathy, including inflammation, fibrosis, and endothelial dysfunction (44, 45), we evaluated the levels of 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress marker, in the kidney tissue and urine, as well as the expression of Il-6 and Tgfβ genes. Although there were no significant differences between the groups on TAM(10d), possibly because of the absence of excess oxidative stress, IHC and urine analyses revealed that 8-OHdG levels were decreased in c-MafΔTAM mice on STZ(12w) TAM(8w) compared with those in c-Maffl/fl mice (Figure 6, H and I). In addition, the expression of Il-6 and Tgfβ genes was reduced in c-MafΔTAM mice (Figure 6, J and K). Together, these data suggest that c-Maf deletion reduces oxidative stress under diabetic conditions, as well as the expression of Il-6, Tgfβ, Col1a1, Col4a3, and fibronectin mRNA, thereby suppressing the inflammatory reaction and fibrotic process.
Finally, we investigated the role of c-Maf in podocytes and immune cells in DKD and the immunological consequences of c-Maf deficiency (46, 47) by staining macrophages expressing c-Maf and WT1 in kidney podocytes on STZ(12w) TAM(8w) and examining the expression of Il-10 and Il-17 genes on TAM(10d) and STZ(12w) TAM(8w). Costaining for macrophages and c-Maf verified the infiltration of macrophages expressing c-Maf into the kidneys of both c-Maffl/fl and c-MafΔTAM mice (Supplemental Figure 5E). However, there were no significant differences in the numbers of WT1-positive nuclei or in the expression of Il-10 and Il-17 genes between the 2 groups (Supplemental Figure 5, F–K). RNA-Seq analysis revealed that the gene expression of c-Mip, which plays an important role in podocyte function and survival (48) and is strongly related to Wilms tumor protein (WT1), did not differ between c-Maffl/fl and c-MafΔTAM mice. Taken together, these results suggest that c-Maf loss exerts renoprotective effects against diabetic kidney injury and that these effects are not mediated through c-Maf loss in macrophages, WT1, c-Mip, IL-10, or IL-17.
In this study, we demonstrate that c-Maf directly regulates the expression of Sglt2 and Glut2 by binding to MARE sites and that c-Maf deletion attenuates STZ-induced diabetic nephropathy by improving diabetic conditions and reducing oxidative stress in the kidney.
The transcription factor c-Maf has been widely studied and has been reported to play a critical role in embryonic kidney development; however, the postnatal functions of c-Maf in adult kidneys remain unknown as c-Maf–null C57BL/6J mice exhibit embryonic lethality. In this study, we demonstrate that c-Maf directly regulates the expression of the glucose transporter genes, Sglt2 and Glut2, thereby, lowering blood glucose levels under diabetic conditions. In addition, we demonstrate that c-Maf deletion can promote recovery from hyperglycemia and glomerular hyperfiltration by downregulating Sglt2 and Glut2, which could be a key mechanism underlying improved kidney function. Moreover, c-MafΔTAM mice showed reduced renal oxidative stress levels and Col1a1, Col4a3, fibronectin, Il-6, and Tgfβ mRNA expression, which may suppress the inflammatory reaction and fibrotic processes as the renal levels and expression of these genes are associated with mesangial cell proliferation, tubular atrophy, and kidney fibrosis (44, 45, 49–51). Therefore, these results suggest that c-Maf deletion improves diabetic conditions and has renoprotective effects.
However, the effects of whole-body c-Maf deletion on other organs that are involved in insulin and glucagon secretion and on several immune cells that are regulated by c-Maf (52, 53) must be considered as must be the limitations of using the STZ diabetic mouse model. The IHC staining in the pancreas and liver showed that there was no c-Maf expression in islet cells and hepatocytes. In addition, because there was no significant change in food intake between the c-Maffl/fl and c-MafΔTAM groups in STZ experiments, c-Maf deletion in the brain has less effect on Glut2, which plays a role in the regulation of food intake and body energy stores (54). Furthermore, from qPCR and IHC staining results, we found that factors, such as macrophages, IL-10, IL-17, WT1, and c-Mip, which are related to c-Maf regulation, may not be affected by c-Maf deficiency, at least in the kidney. Taken together with the fact that there were no significant changes in blood insulin, glucagon, and IPGTT in c-MafΔTAM mice on TAM(10d) compared with that in c-Maffl/fl mice, under conditions of this study, c-Maf deletion had almost no effect on Glut2 expression and insulin and glucagon secretion in the pancreas, liver, and brain and in several immune cells that are regulated by c-Maf.
Second, the STZ diabetic mouse model, although well accepted, has its limitations due to the cytotoxic effect of STZ on proximal tubular cells. In addition, STZ enters the cells through Glut2, and its cytotoxic effect also influences proximal tubular cells; hence, it has to be considered that similar albumin levels may reflect the damage caused by STZ to proximal tubules, regardless of the reduction in albumin transporter levels. In this study, to minimize the nonspecific toxicity of STZ on kidney proximal tubules in the C57BL/6 background mice, both multiple and low-dose injections of STZ were utilized (55, 56). By minimizing its toxicity, the effect on megalin function was minimized, resulting in the maintenance of albumin reabsorption. In fact, urinary albumin levels in megalin-knockout mice increased substantially, 2 weeks after TAM administration compared with the levels before TAM administration (8 weeks after STZ), whereas urinary albumin levels in megalinfl/fl mice increased before and after TAM administration. In other words, the maintenance of the megalin function resulted in the maintenance of albumin reabsorption, which suggests the amelioration of the amount of urinary albumin excretion in c-MafΔTAM mice compared with that in c-Maffl/fl mice.
In addition to the STZ diabetic mouse model, db/db mice of the C57BLKS/J strain are frequently used as a model of early to moderately advanced morphological changes in the kidney that are associated with diabetic nephropathy. Indeed, a proof of protective effects of c-Maf deletion not only in type 1 but also in type 2 diabetes using the mouse model can strengthen the notion of a close interaction between c-Maf and Sglt2. However, one of the main purposes of this study was to detect whether c-Maf deletion has protective effects on severe diabetic nephropathy caused by hyperglycemia. Numerous studies have reported that db/db mice show an increase in glomerular basement thickening, podocyte loss, moderate mesangial matrix expansion, oxidative stress, and inflammation in the kidneys, but they did not develop features of severe tubulointerstitial fibrosis. In contrast to db/db mice, although the STZ-induced C57BL/6 mouse model is milder than the models in other strains, these mice exhibit advanced renal pathological changes, including glomerular hypertrophy, excess oxidative stress, and inflammation, which are strongly implicated in the pathogenesis of diabetic nephropathy, resulting in severe tubulointerstitial fibrosis (57). For this reason, we selected STZ-induced C57BL/6 mice; in fact, c-Maffl/fl mice showed severe tubulointerstitial fibrosis, and c-MafΔTAM mice improved diabetic nephropathy as a result of c-Maf deletion. Therefore, we identified the high possibility of an important potentially novel function of c-Maf in adult kidneys and demonstrated that c-Maf deletion in the kidney exerts beneficial effects on diabetic nephropathy. In the future, db/db mice can be used as a useful model to observe the effects of c-Maf–deficient Sglt2 inhibition on multiple organs.
Although no previous studies have demonstrated that c-Maf is a major transcription factor regulating the expression of glucose transporters in kidney proximal tubules, we found that c-Maf directly regulates Sglt2 and Glut2, which are responsible for the reabsorption of renally filtered glucose in proximal tubules. It has previously been reported that the transcription factors Hnf1α, Hnf3β, and Srebp1c link Sglt2 expression (20, 22, 58, 59–61), while Hnf4α-mutant mice present with renal glycosuria due to the loss of Sglt2 (61, 62). Furthermore, Sp1 has been shown to promote Sglt1 and Sglt2 expression in the presence of Zn (63). Together, these studies indicate that these transcription factors play key roles in mammalian glucose homeostasis. Consistently, we found that the kidney glycosuria phenotype correlated with the downregulated expression of these transporters, indicating that c-Maf is a major transcription factor that regulates transporter expression in kidney proximal tubules. Interestingly, in the previous report, renal Glut2 deficiency reduced the expression of Sglt2 14 days after the third TAM injection via downregulation of the transcription factor HNF1α (20). In contrast, in this study, c-Maf deletion substantially decreased the expression of Sglt2 on only a day after the second TAM injection. Therefore, the time lag in the reduction of Sglt2 expression between previous studies and this study suggested direct regulation of Sglt2 by c-Maf. Furthermore, considering the lack of significant differences in Hnf1a expression, and the results of ChIP and Luc assays, we concluded that c-Maf directly controls the expression of Sglt2 and Glut2 in the kidney, respectively. In a future study, the cooperative functions of c-Maf and other transcription factors should be explored.
Our results also indicate a possibility that c-Maf is a potentially novel therapeutic target gene for diabetic nephropathy. SGLT2 blockades are considered the most influential method for improving diabetes. Here, we demonstrate that c-Maf deletion suppressed the development of not only hyperglycemia and hyperfiltration but also diabetic nephropathy, similar to the treatment with SGLT2 inhibitors in mice (40–43). In contrast, a previous study reported that Sglt2-knockout mice with diabetic nephropathy displayed attenuated hyperglycemia and glomerular hyperfiltration, but not kidney injury (35). The phenotypic differences between our study and the previous study using Sglt2-knockout mice suggest the presence of other factors as well as SGLT2 inhibitor treatment that attenuate diabetic nephropathy in c-MafΔTAM mice.
After the discovery of SGLT2 inhibitors, Slc-mediated membrane transporters attracted increasing attention as new drug discovery targets. For example, prototypical loop diuretics, such as furosemide, bumetanide, and torsemide, can bind to chloride cotransporters (NKCCs: SLC12A1) and block ion transport directly and can, therefore, be used to treat heart failure, liver scarring, or kidney disease (64). Ezetimibe targets the Niemann–Pick C1–like 1 (NPC1L1: SLC65A2) protein, thereby reducing cholesterol absorption from the intestine (65). Recently, other Slc-mediated transporters have attracted attention as potential novel therapeutic targets. For instance, the inhibition of Glut2 (Slc2a2) dynamics has the potential to improve diabetes mellitus in mice (21), and Slc7A5, a glutamine antiporter, could be an attractive target for therapy-resistant, KRAS-mutant colorectal cancer in mice (66). In addition to Slc-mediated transporters, non–Slc-mediated transmembrane transporters have potential as therapeutic agents. Tolvaptan, a competitive vasopressin receptor 2 antagonist, has been used to treat hyponatremia associated with congestive heart failure, cirrhosis, and the syndrome of inappropriate antidiuretic hormone (67). In this study, c-MafΔTAM mice excreted higher urinary albumin levels on TAM(10d) than did c-Maffl/fl mice due to the downregulation of the expression of megalin and cubilin genes. Previous studies have reported that albumin reabsorption in the proximal tubules promotes renal damage and that proximal tubular proteinuria reduces the tubular burden (68–70). Therefore, it is conceivable that various Slc- and non–Slc-mediated transmembrane transporters could be potential therapeutic agents for various diseases. Indeed, we demonstrate a strong relationship between c-Maf and Slc-mediated transporters, such as glucose transporters and amino acid transporters (Figure 2A), indicating that c-Maf may play an important role in controlling not only the transporters that are known therapeutic agents but also other unknown transporters. In fact, a previous study reported that by suppressing the cellular excess of amino acids, CB1R maintains the normal activity of mTORC1, which may help ameliorate DKD (22). Therefore, the presence of amino acid transporters as a drug target controlled by c-Maf can be an advantage of c-Maf inhibition/deletion over SGLT2 inhibition. Although, in the future, it will be important and necessary to clarify the relationship between c-Maf and these transporters, c-Maf has potential as a comprehensive therapeutic target in the treatment of not only diabetic nephropathy but also other diseases.
The limitations of targeting c-Maf specifically in the kidney must be considered. Currently, treatment of CKD requires drugs, such as hormones and nonsteroidal antiinflammatory drugs, to be delivered at high concentrations into the kidney, which results in adverse systemic reactions because of the nondiscriminatory distribution of the drugs to sites other than the kidneys (71). Therefore, this can presently be a limitation of targeting c-Maf, specifically in the kidney. However, the concurrent rapid development of nanotechnology and macromolecular carrier technology and emerging preclinical evidence have greatly facilitated advancements in kidney-targeted drug delivery systems. These systems have offered the possibility to plan a cell-targeted administration of new and traditional drugs (72, 73). There are several hurdles, such as solubility, toxicity, and suboptimal renal targeting, which slow the transition toward clinical applications. While renal drug delivery technologies are still in the preclinical stage, the efficacy of a wide array of therapeutics delivered using the diverse drug delivery platforms has been demonstrated by in vitro studies and through in vivo testing in animal models. Therefore, emerging preclinical evidence supports the feasibility of a drug delivery technology for the treatment of kidney diseases with different etiologies and has been offering novel platforms and tools that foster opportunities for the gradual development of new treatments (74).
In conclusion, this study elucidates potentially unique mechanisms of c-Maf gene regulation as well as the renoprotective effects of c-Maf deletion under diabetic conditions through the regulation of Sglt2 and Glut2 in the kidneys. Considering the strong association between c-Maf and these novel therapeutic target genes for diabetes and diabetic nephropathy, our research contributes toward novel advances in basic and clinical investigation.
Animals. All animals were maintained under specific pathogen–free conditions at the Laboratory Animal Resource Center of the University of Tsukuba, Ibaraki, Japan. All experiments were performed according to the guidelines of the Care and Use of Laboratory Animal Resource Center at the University of Tsukuba and were approved by its Institutional Review Board. CAG-Cre-ERTM transgenic mice were purchased from the Jackson Laboratory to generate c-Maf–conditional knockout mice (c-Maffl/fl; CAG-Cre-ERTM) (75). c-Maffl/fl mice (c-Maffl/fl) containing loxP sites were generated as reported previously (11). To activate the Cre recombination system, 8-week-old c-Maffl/fl; CAG-Cre-ERTM mice were injected intraperitoneally with 75 mg TAM/kg for 5 consecutive days. These mice are herein referred to as “c-MafΔTAM” mice. c-Maffl/fl control mice were also treated with TAM. TAM (T5648-1G; MilliporeSigma) was first dissolved in ethanol and then mixed with corn oil as described previously (76). In addition, 6-week-old mice with the β-galactosidase (LacZ) gene inserted into the c-Maf locus were used to visualize c-Maf accumulation in the kidneys (5). Type 1 diabetes was induced in the mice through consecutive intraperitoneal injections with STZ (195-15154; FUJIFILM Wako Pure Chemical Corporation; 50 mg/kg body weight), freshly prepared in 0.05 M sodium citrate buffer (pH 4.5), for 5 days after 5–6 hours of fasting. Blood glucose levels were measured 3 weeks after the last STZ injection to identify mice with levels > 300 mg/dL. Mice with this level were considered diabetic and were used in subsequent experiments. Food intake was measured for 24 hours. The detailed experimental protocol is provided in Supplemental Figure 4A.
Urine and serum analysis. Mice were housed in metabolic cages for 24 hours, and urine samples were then collected from each mouse. The urine samples were placed on ice during collection and then stored at −30°C. Urinary levels of 8-OHdG and other urinary components were evaluated by the Japan Institute for the Control of Aging, Nikken SEIL, and the Oriental Yeast Corporation, respectively. Amino acids were analyzed using an Automatic Amino Acid Analyzer (JEOL JLC-500/V2) by Tamaki Hirose at Chemical Analysis Division, Research Facility Center for Science and Technology, University of Tsukuba. Serum amino acid levels were evaluated by the Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan. Water intake was measured using a specific bottle (Shinfactory). Serum samples were stored at about 25°C for approximately 15 minutes followed by 2–4 hours on ice, centrifuged at 1,450g for 10 minutes at 15°C, and frozen at −30°C. Serum components were evaluated by the Oriental Yeast Corporation. Serum insulin and glucagon levels were examined using an Ultra-Sensitive Mouse Insulin ELISA Kit (M1108; Morinaga) and a Glucagon ELISA Kit (10-1281-01; Mercodia), respectively.
IPGTT. After 10–12 hours of fasting, blood samples were collected from the tail vein, and basal blood glucose levels were measured at 0 minutes. The mice were then intraperitoneally injected with a glucose solution (20% glucose and 2% of glucose/kg body mass) using an Otsuka Glucose Injection 50% (M7I73) diluted in saline, and blood glucose levels were measured, 15, 30, 60, and 120 minutes after injection, using the Medisafe FIT Blood Glucose Meter (TERUMO).
qPCR. Total RNA was extracted from the kidneys of c-MafΔTAM and c-Maffl/fl mice using the ISOGEN reagent (311-02501; Nippon Gene). cDNA was synthesized using a QuantiTect reverse transcription kit (205313; QIAGEN) according to the manufacturer’s protocol. qPCR was performed using a Thermal Cycler Dice real-time system (TaKaRa) with THUNDERBIRD SYBR qPCR Mix (QPS-201; Toyobo). The primers listed in Supplemental Table 3 were used to amplify and detect the target genes. The expression of target genes was normalized to that of Hprt.
Histopathological analysis. IHC analysis was performed as described previously (77), with some modifications, in 3-, 6-, or 8-week-old mice to detect c-Maf, Sglt2 and Glut2, β-galactosidase, 8-OHdG, albumin, macrophages, and WT1. Briefly, kidneys were collected from c-MafΔTAM and c-Maffl/fl mice, fixed in 10% Formalin Neutral Buffer Solution (133-10311; FUJIFILM Wako Pure Chemical Corporation) at 4°C for 24 hours, embedded in paraffin, cut into 3 μm–thick sections, and placed on slides. After removal of paraffin, the tissue sections were washed with phosphate-buffered saline (PBS), then subjected to antigen retrieval (S1699; Dako) for 1 hour at 90°C for c-Maf, Sglt2, Glut2, β-galactosidase, 8-OHdG, albumin, macrophages, and WT1. Approximately 30 minutes after being cooled to about 25°C, the samples were washed with PBS and permeabilized with 0.3% Triton X-100/PBS to detect β-galactosidase; this process was skipped for c-Maf, Sglt2, Glut2, albumin, macrophage, and WT1 detection. The samples were then blocked for 1 hour in PBS with 5% bovine serum albumin (BSA) and 10% goat serum, then incubated for 40 hours at 4°C with the following primary antibodies: anti–c-Maf rabbit monoclonal antibody (1:200; M00654; Boster Biological Technology), anti-Sglt2 (1:200; ab85626; Abcam), anti-Glut2 (1:200; 600-401-GN3; ROCKLAND), rabbit anti–β-galactosidase (1:200; 4600-1409; Biogenesis), anti-albumin rabbit polyclonal antibodies (1:200; GTX102419; GeneTex), anti-macrophage rat monoclonal antibodies (RM0029-11H3) (1:200; ab56297; Abcam), and anti-WT1 rabbit monoclonal antibodies [CAN-R9(IHC)-56-2] (1:300; ab89901; Abcam). To detect β-galactosidase, c-Maf, Sglt2, Glut2, macrophages, and WT1, samples were washed with PBS, then stained with Histofine Simple Stain MAX-PO (414341; Nichirei Biosciences), and signals were amplified using a Histofine DAB substrate kit (425011; Nichirei Biosciences). The sections were mounted with Fluoromount (Diagnostic BioSystems). To detect 8-OHdG, samples were deparaffinized and subjected to antigen retrieval for 10 minutes at 121°C. Approximately 30 minutes after cooling at 23°C–26°C, the samples were blocked for 1 hour in PBS with 5.0% BSA and incubated for 40 hours at 4°C with anti–8-OHdG monoclonal antibodies (N45.1; 1:200; JaICA, Nikken SEIL). After washing with PBS, samples were stained with Histofine Simple Stain AP(M) (414251; Nichirei Biosciences) for signal amplification, and color signals were amplified using a Histofine Alkaline Phosphatase Detection Kit (415261; Nichirei Biosciences). All images were acquired using a BIOREVO BZ-9000 microscope (Keyence). For electron microscopy, tissues were fixed with 2.5% glutaraldehyde in PBS. Standard methods were used to perform transmission electron microscopy.
Measurement of PAMS- and Sirius red–positive area and the number of WT1-positive nuclei. The PAMS-positive area was detected as described previously (78), with some modifications. The degree of mesangial matrix expansion, tubulointerstitial fibrogenesis, and the number of WT1-positive nuclei were assessed by measuring the PAMS-positive and mesangial Sirius red–positive areas and by performing IHC staining for WT1 in the kidneys. PAMS-stained glomeruli (20–25 fields at 40× original magnification), Sirius red–stained interstitial lesions (20–27 fields at 20× original magnification), and the number of WT1-positive nuclei (15–20 fields at 40× original magnification) were evaluated in each mouse using a BIOREVO BZ-9000 microscope. Glomerular size (tuft area) was measured by tracing the tuft (red color), while the mesangial matrix area was defined as the PAMS-positive area (green color within the tuft area). The mesangial matrix index represents the ratio of the mesangial matrix area to the tuft area. Tubulointerstitial lesions, defined as Sirius red–positive areas (blue color), were measured to detect fibrogenesis and expressed as a percentage of the total area. The number of WT1-positive nuclei (green color) was counted within the tuft area and divided by the area of the glomerulus.
ChIP assay. The ChIP Assay Kit protocol (17-295; MilliporeSigma) was modified based on a previous study (79). Briefly, kidneys extracted from c-MafΔTAM and c-Maffl/fl mice were fixed in 1% paraformaldehyde at 4°C for 24 hours and stored at −80°C until use. The samples were incubated for 10 minutes in ChIP lysis buffer (50 mM Tris-HCl [pH 7.9], 10 mM ethylenediaminetetraacetic acid [EDTA], 0.1% sodium dodecyl sulfate [SDS], and 1 mM phenylmethylsulfonyl fluoride [PMSF]) and sonicated to shear the chromatin into 500–1,000 bp DNA fragments. After centrifugation at 15,000 rpm for 10 minutes at 4°C, the supernatant was collected and incubated overnight at 4°C with rotation in ChIP dilution buffer (16.7 mM Tris-HCl [pH 7.9], 16.7 mM NaCl, 1.2 mM EDTA, 1.1% Triton X-100, 0.01% SDS, and 1.1 mM PMSF) and Protein A/BSA/ssDNA (Protein A beads, 10 mM Tris-HCl [pH 7.9], 1.0 mM EDTA, 0.1% Triton X-100, 0.05% NaN3, 0.5 mg/mL BSA, and 0.2 mg/mL salmon sperm DNA). Samples were then centrifuged at 730g for 5 minutes at 4°C and the supernatant was collected. Two-thirds of the lysates were incubated with anti-H3 antibodies (ab1791; Abcam), c-Maf antibodies (A300-613A; Bethyl), and normal rabbit IgG (12-370; MilliporeSigma) with rotation at 4°C overnight. One-third of the lysates were used as input samples. After incubation, Protein A/BSA/ssDNA was added, and the samples were incubated at 4°C for 1 hour. After centrifugation at 730g for 5 minutes at 4°C, the supernatant was discarded, and the antibody–protein–DNA complexes were washed twice with a high-salt wash buffer (20 mM Tris-HCl [pH 7.9], 500 mM NaCl, 2 mM EDTA, 1% Triton X-100, and 0.1% SDS), once with LiCl wash buffer (10 mM Tris-HCl [pH 7.9], 250 mM LiCl, 1 mM EDTA, 1% NP-40, and 1% sodium deoxycholate), and twice with Tris-EDTA (pH 7.9). The samples were then incubated in an elution buffer (100 mM NaHCO3, 1% SDS, and 10 mM dithiothreitol) at about 25°C for 15 minutes, then centrifuged at 730g for 1 minute at about 25°C, and the supernatant was collected. This elution process was repeated in duplicate. The samples and input samples were incubated at 65°C overnight with an elution buffer and 5 M NaCl, then treated with 1 M Tris-HCl (pH 6.2), 0.5 M EDTA, and 20 μg/mL Proteinase K (29442-85; Nacalai Tesque) at 37°C for 1 hour, and then DNA was extracted using phenol:chloroform:isoamyl alcohol and precipitated using ethanol. DNA fragments were subjected to qPCR using KOD SYBR qPCR Mix (QKD-201; Toyobo) with the specific primer sets listed in Supplemental Table 1.
Dual-luciferase assays and site-directed mutagenesis. Sglt2 and Glut2 sequences including the MARE (−1,019 to −53 bp and −303 to 287 from transcription start site, respectively) were generated using PCR with the primers listed in Supplemental Table 3. These products were digested using XhoI and KpnI and inserted upstream of luc2CP in the pGL4.28 vector. Clones were confirmed using sequencing. Site-directed mutagenesis of Sglt2 and Glut2 was performed using a KOD-Plus- Mutagenesis Kit (SMK-101; Toyobo) with the primers listed in Supplemental Table 3. For the luciferase assay, HEK293T cells purchased from ATCC were seeded in 24-well plates, incubated for 17–24 hours, and then cotransfected with pGL4.28 containing the inserted Sglt2 and Glut2 sequences (100 ng/μL) and PEFX containing the inserted c-Maf sequence (0, 10, 30, or 90 ng/μL). Renilla luciferase signals were normalized to pRL-TK (50 ng/μL for Sglt2, Glut2), and GFP was used to confirm successful transfection. A total of 300 ng of vectors were added to each well. These experiments were performed 3 times in triplicate. Dual-luciferase assays were performed using the Dual-Luciferase Reporter Assay System (E1910; Promega). After transfection for about 24 hours for Sglt2 and Glut2, the cells were lysed, and 20 μL cell lysate was mixed with 100 μL Luciferase Assay Reagent II. Samples were measured using a GloMax 20/20 Luminometer (E5311; Promega) and again after Stop & Glo reagent (100 μL) was added to each sample.
RNA-Seq transcriptome analysis. Total RNA was isolated using the TRIzol reagent (Thermo Fisher Scientific). RNA quality was examined using an RNA 6000 Pico Kit (Agilent). A total of 50 ng RNA was used for the preparation of the RNA-Seq library using an NEB Next rRNA Depletion Kit and an NEB Next Ultra Directional RNA Library Prep Kit (New England Biolabs). The 2 × 36 base paired-end sequencing was performed by Tsukuba i-Laboratory LLP using NextSeq 500 technology (Illumina). FASTQ files were imported into the CLC Genomics Workbench (Version 10.1.1; QIAGEN). Sequence reads were mapped to the mouse reference genome (mm10). Gene expression values were calculated using the quantile method. Gene expression was compared between c-Maffl/fl control mice and c-MafΔTAM mice using the Empirical Analysis DGE tool (edgeR test) in the CLC Genomics Workbench. False discovery rate (FDR) adjustments (80) were applied to correct for multiple testing, and DEGs were identified using an FDR cutoff of 0.05. The volcano plots were generated using a custom R script. Pathway analysis was performed using Metascape (81). All custom scripts for RNA-Seq analysis are available at https://github.com/akikuno/Maf_cKO_Kidney (commit ID 27dca2d). RNA-Seq data in this paper are available in NCBI Gene Expression Omnibus, accession number GSE199916. Heatmaps were generated using Python3 and Morpheus (https://software.broadinstitute.org/morpheus/).
Statistics. All data were presented as the mean and the standard error of the mean. To assess whether differences between c-MafΔTAM and c-Maffl/fl mice were statistically significant, a minimum of 3 biological replicates were analyzed using Welch’s t test, and a P < 0.05 was considered significant. Holm-Šídák corrections were applied for multiple statistical tests in Figure 4, D and H; Figure 5A; and Supplemental Figure 4J.
Study approval. All experiments were performed according to the guidelines of the Care and Use of Laboratory Animal Resource Center at the University of Tsukuba and were approved by its Institutional Review Board.
ST supervised the study. MF designed the experiments. MF and MO performed the experiments. MF and AK detected MARE sites on Sglt2 and Glut2. MF analyzed data and drafted the manuscript. MF, TH, SI, and AK analyzed RNA-Seq data. SK analyzed serum amino acids. MF, NM, TH, SI, MO, AK, SK, KY, and ST edited and revised the manuscript. All authors interpreted the experimental results and approved the final version of the manuscript.
This study was supported by the Uehara Memorial Foundation, JSPS KAKENHI grant number JP19H00966, the Princess Takamatsu Cancer Research Fund, and grant number JPMJPF2017 from the Japan Science and Technology Agency.
We thank Masayuki Yamamoto at Tohoku University for providing the pGL4.28 vector; Kosuke Kato at the University of Tsukuba for lectures on the ChIP assay; and Tamaki Hirose at Chemical Analysis Division, Research Facility Center for Science and Technology at the University of Tsukuba, and Megumi Takemura at the University of Tsukuba for designing the graphical abstract and PhD Program in Human Biology at the University of Tsukuba. We would like to thank Editage (https://www.editage.com) for English language editing.
Address correspondence to: Mitsunori Fujino or Satoru Takahashi, Laboratory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan. Phone: 81.29.853.7516; Email: s1930551@s.tsukuba.ac.jp (MF); satoruta@md.tsukuba.ac.jp (ST).
Conflict of interest: The authors have declared that no conflict of interest exists.
Copyright: © 2023, Fujino et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.
Reference information: JCI Insight. 2023;8(6):e163306.https://doi.org/10.1172/jci.insight.163306.