[HTML][HTML] Biophysical characteristics of the pig kidney Na+/glucose cotransporter SGLT2 reveal a common mechanism for SGLT1 and SGLT2
B Mackenzie, DDF Loo… - Journal of Biological …, 1996 - ASBMB
The Na+-dependent, low affinity glucose transporter SGLT2 cloned from pig kidney is 76%
identical (at the amino acid level) to its high affinity homologue SGLT1. Using two-
microelectrode voltage clamp, we have characterized the presteady-state and steady-state
kinetics of SGLT2 expressed in Xenopus oocytes. The kinetic properties of the steady-state
sugar-evoked currents as a function of external Na+ and α-methyl-D-glucopyranoside (αMG)
concentrations were consistent with an ordered, simultaneous transport model in which Na+ …
identical (at the amino acid level) to its high affinity homologue SGLT1. Using two-
microelectrode voltage clamp, we have characterized the presteady-state and steady-state
kinetics of SGLT2 expressed in Xenopus oocytes. The kinetic properties of the steady-state
sugar-evoked currents as a function of external Na+ and α-methyl-D-glucopyranoside (αMG)
concentrations were consistent with an ordered, simultaneous transport model in which Na+ …