(A) Acquired IMC images are visualized in representative pseudocolor plots and histogram with overlays to show that HepPar1⁺ events can be gated separately from CD45⁺ events, and that CD45⁺ events are enriched for immune markers (e.g., CD3, CD8, CD68). Far right contour plot overlay shows that CD68⁺CD45⁺ gate (red) is enriched for concurrent CD16/CD14 expression, compared with CD68^–CD45⁺ gate (blue). (B) Representative contour plot and histogram overlays provide comparisons for portal (red) versus lobular (blue) detection of HepPar1, CD45, and/or CD3 expression, with expected lobular but not portal detection for HepPar1⁺ hepatocytes and both lobular and portal detection for CD45⁺ or CD3⁺ immune cells. (C) Overlay of lobular CD45^–HepPar1⁺ (green), lobular CD45⁺HepPar1^– (purple), and all portal (orange) events show HBsAg expression limited to CD45^–HepPar1⁺ cells, CK19 expression limited to portal cells, and CD68 expression in both lobular CD45⁺HepPar1^– immune cells and portal cells. (D) Tissue image reproduced with portal and/or lobular location of bile ducts, hepatocytes, and immune cells by applying x/y spatial coordinates to analyzed IMC data. (E) Scatter plots comparing %portal/total area, ALT, HBV DNA, and age relative to total, lobular, and portal CD45⁺ cell density per mm² for 28 IA (red diamond) and 6 IT (blue X) subjects. A single outlier with portal CD45⁺ immune cell density at 43,365 was not shown graphically, although it was included in calculating the Spearman’s correlation and P values (shown in red fonts for P < 0.05). (F) Scatter plots comparing histological scores (Ishak lobular inflammation, Ishak portal inflammation, Ishak periportal hepatitis [piecemeal necrosis], Ishak fibrosis, and perisinusoidal fibrosis scores) with total, lobular, and portal CD45⁺ cell density per mm² for 28 IA (red diamond) and 6 IT (blue X) subjects, with Spearman’s correlations and P values.