Citations to this article
Line Hjort, … , Richard Saffery, Allan Arthur Vaag
Published September 6, 2018
Citation Information: JCI Insight. 2018;3(17):e122572. https://doi.org/10.1172/jci.insight.122572.
Citation Information: JCI Insight. 2018;3(17):e122572. https://doi.org/10.1172/jci.insight.122572.
Abstract
Offspring of women with gestational diabetes mellitus (GDM) are at increased risk of developing metabolic disease, potentially mediated by epigenetic mechanisms. We recruited 608 GDM and 626 control offspring from the Danish National Birth Cohort, aged between 9 and 16 years. DNA methylation profiles were measured in peripheral blood of 93 GDM offspring and 95 controls using the Illumina HumanMethylation450 BeadChip. Pyrosequencing was performed for validation/replication of putative GDM-associated, differentially methylated CpGs in additional 905 offspring (462 GDM, 444 control offspring). We identified 76 differentially methylated CpGs in GDM offspring compared with controls in the discovery cohort (FDR, P < 0.05). Adjusting for offspring BMI did not affect the association between methylation levels and GDM status for any of the 76 CpGs. Most of these epigenetic changes were due to confounding by maternal prepregnancy BMI; however, 13 methylation changes were independently associated with maternal GDM. Three prepregnancy BMI–associated CpGs (cg00992687 and cg09452568 of ESM1 and cg14328641 of MS4A3) were validated in the replication cohort, while cg09109411 (PDE6A) was found to be associated with GDM status. The identified methylation changes may reflect developmental programming of organ disease mechanisms and/or may serve as disease biomarkers.
Authors
Line Hjort, David Martino, Louise Groth Grunnet, Haroon Naeem, Jovana Maksimovic, Anders Henrik Olsson, Cuilin Zhang, Charlotte Ling, Sjurdur Frodi Olsen, Richard Saffery, Allan Arthur Vaag
Total citations by year
Citation information
Citations to this article (63)
| Title and authors | Publication | Year |
|---|---|---|
|
Longitudinal DNA methylation profiles in saliva of offspring from mothers with gestational diabetes: associations with early childhood growth patterns
Linares-Pineda TM, Lendínez-Jurado A, Piserra-López A, Suárez-Arana M, Pozo M, Molina-Vega M, Picón-César MJ, Morcillo S |
Cardiovascular Diabetology | 2025 |
|
Healthy lifestyle before and during pregnancy to prevent childhood obesity: study protocol for a parallel group randomised trial — the PRE-STORK trial
Kornerup N, Danielsen JH, Sahl RE, Pico ML, Johansen MY, Knop FK, Bønnelykke K, Bergholt T, Kelstrup L, Foghsgaard S, Ghauri N, Grønlund E, Lund L, Vinter CA, Lyng Forman J, Barrès R, Kragelund Nielsen K, Andersen A, Torekov SS, Groth Grunnet L, Vilsbøll T |
BMJ Open | 2025 |
|
Gestational diabetes induces a lasting hematopoietic memory that impacts the long-term health of the offspring
Vinothini Govindarajah, Masahide Sakabe, Samantha Good, Michael Solomon, Ashok Arasu, Nong Chen, Ady Kendler, Mei Xi, Damien Reynaud |
Journal of Clinical Investigation | 2024 |
|
Maternal obesity induced metabolic disorders in offspring and myeloid reprogramming by epigenetic regulation
Kweon JY, Mun H, Choi MR, Kim HS, Ahn YJ |
Frontiers in Endocrinology | 2024 |
|
Multigenerational diabetes mellitus
Thornton JM, Shah NM, Lillycrop KA, Cui W, Johnson MR, Singh N |
Frontiers in Endocrinology | 2024 |
|
Diabetes Mellitus and Pregnancy: An Insight into the Effects on the Epigenome
Meza-León A, Montoya-Estrada A, Reyes-Muñoz E, Romo-Yáñez J |
Biomedicines | 2024 |
|
Integrative network analysis of differentially methylated regions to study the impact of gestational weight gain on maternal metabolism and fetal-neonatal growth.
Argentato PP, Guerra JVDS, Luzia LA, Ramos ES, Maschietto M, Rondó PHC |
Genetics and molecular biology | 2024 |
|
Substance P Concentration in Gestational Diabetes and Excessive Gestational Weight Gain and Its Impact on Neonatal Anthropometry.
Niebrzydowska-Tatus M, Pełech A, Bień K, Rekowska AK, Domańska A, Kimber-Trojnar Ż, Leszczyńska-Gorzelak B, Trojnar M |
International journal of molecular sciences | 2024 |
|
Epigenetics of the non-coding RNA nc886 across blood, adipose tissue and skeletal muscle in offspring exposed to diabetes in pregnancy.
Hjort L, Bredgaard SS, Manitta E, Marques I, Sørensen AE, Martino D, Grunnet LG, Kelstrup L, Houshmand-Oeregaard A, Clausen TD, Mathiesen ER, Olsen SF, Saffery R, Barrès R, Damm P, Vaag AA, Dalgaard LT |
Clinical Epigenetics | 2024 |
|
Danish Diabetes Birth Registry 2: a study protocol of a national prospective cohort study to monitor outcomes of pregnancies of women with pre-existing diabetes.
Knorr S, Aalders J, Overgaard M, Støvring H, Mathiesen ER, Damm P, Clausen TD, Bjerre-Christensen U, Andersen LLT, Vinter C, Kofoed-Enevoldsen A, Lauenborg J, Kampmann U, Fuglsang J, Ovesen PG, Christensen TT, Sørensen A, Ringholm L, Jensen DM |
BMJ Open | 2024 |
|
D NA methylation in cord blood partially mediates the effects of prepregnancy BMI on early childhood offspring BMI
Maguolo A, Jönsson J, Perfilyev A, Maziarz M, Vaag A, Malchau Carlsen E, Nørgaard K, Franks PW, Renault KM, Ling C |
Obesity (Silver Spring, Md.) | 2024 |
|
Maternal Influences on Offspring Food Allergy
Lee HY, Nazmul T, Lan J, Oyoshi MK |
Immunological reviews | 2024 |
|
Data processing choices can affect findings in differential methylation analyses: an investigation using data from the LIMIT RCT
Louise J, Deussen AR, Dodd JM |
PeerJ | 2023 |
|
Maternal obesity and gestational diabetes reprogram the methylome of offspring beyond birth by inducing epigenetic signatures in metabolic and developmental pathways.
Alba-Linares JJ, Pérez RF, Tejedor JR, Bastante-Rodríguez D, Ponce F, Carbonell NG, Zafra RG, Fernández AF, Fraga MF, Lurbe E |
Cardiovascular Diabetology | 2023 |
|
Exploring the Diet-Gut Microbiota-Epigenetics Crosstalk Relevant to Neonatal Diabetes
Alsharairi NA |
Genes & development | 2023 |
|
Social Determinants of Health and Cerebral Small Vessel Disease: Is Epigenetics a Key Mediator?
Parodi L, Mayerhofer E, Narasimhalu K, Yechoor N, Comeau ME, Rosand J, Langefeld CD, Anderson CD |
Journal of the American Heart Association | 2023 |
|
Gestational diabetes mellitus: genetic factors, epigenetic alterations, and microbial composition.
Lizárraga D, Gómez-Gil B, García-Gasca T, Ávalos-Soriano A, Casarini L, Salazar-Oroz A, García-Gasca A |
Acta Diabetologica | 2023 |
|
Neurodevelopmental Programming of Adiposity: Contributions to Obesity Risk
Skowronski AA, Leibel RL, LeDuc CA |
Endocrine reviews | 2023 |
|
Epigenetics of pregnancy: looking beyond the DNA code
D Zuccarello, U Sorrentino, V Brasson, L Marin, C Piccolo, A Capalbo, A Andrisani, M Cassina |
Journal of Assisted Reproduction and Genetics | 2022 |
|
Hepatic IGF2/H19 Epigenetic Alteration Induced Glucose Intolerance in Gestational Diabetes Mellitus Offspring via FoxO1 Mediation.
Jiang Y, Zhu H, Chen Z, Yu YC, Guo XH, Chen Y, Yang MM, Chen BW, Sagnelli M, Xu D, Zhao BH, Luo Q |
Frontiers in Endocrinology | 2022 |
|
Effect of an antenatal diet and lifestyle intervention and maternal BMI on cord blood DNA methylation in infants of overweight and obese women: The LIMIT Randomised Controlled Trial
Louise J, Deussen AR, Koletzko B, Owens J, Saffery R, Dodd JM |
PloS one | 2022 |
|
An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective.
Dvoran M, Nemcova L, Kalous J |
Biomedicines | 2022 |
|
DNA Methylation and Gene Expression in Blood and Adipose Tissue of Adult Offspring of Women with Diabetes in Pregnancy-A Validation Study of DNA Methylation Changes Identified in Adolescent Offspring.
Manitta E, Fontes Marques IC, Stokholm Bredgaard S, Kelstrup L, Houshmand-Oeregaard A, Dalsgaard Clausen T, Groth Grunnet L, Reinhardt Mathiesen E, Torp Dalgaard L, Barrès R, Vaag AA, Damm P, Hjort L |
Biomedicines | 2022 |
|
Epigenetic and transcriptomic alterations in offspring born to women with type 1 diabetes (the EPICOM study).
Knorr S, Skakkebæk A, Just J, Johannsen EB, Trolle C, Vang S, Lohse Z, Bytoft B, Damm P, Højlund K, Jensen DM, Gravholt CH |
BMC Medicine | 2022 |
|
Maternal obesity in pregnancy and children's cardiac function and structure: A systematic review and meta-analysis of evidence from human studies.
den Harink T, Roelofs MJM, Limpens J, Painter RC, Roseboom TJ, van Deutekom AW |
PloS one | 2022 |
|
Gestational diabetes mellitus, epigenetic age and offspring metabolism
Kim C, Harrall KK, Glueck DH, Needham BL, Dabelea D |
Diabetic Medicine | 2022 |
|
Maternal High-Fat Diet Promotes Abdominal Aortic Aneurysm Expansion in Adult Offspring by Epigenetic Regulation of IRF8-Mediated Osteoclast-like Macrophage Differentiation
M Saburi, H Yamada, N Wada, S Motoyama, T Sugimoto, H Kubota, D Miyawaki, N Wakana, D Kami, T Ogata, S Matoba |
Cells | 2021 |
|
Epigenetic Regulation of Adipogenesis in Development of Metabolic Syndrome
R Pant, P Firmal, VK Shah, A Alam, S Chattopadhyay |
Frontiers in Cell and Developmental Biology | 2021 |
|
Maternal Low-Grade Chronic Inflammation and Intrauterine Programming of Health and Disease
F Parisi, R Milazzo, VM Savasi, I Cetin |
International journal of molecular sciences | 2021 |
|
Aberrant DNA Methylation Mediates the Transgenerational Risk of Metabolic and Chronic Disease Due to Maternal Obesity and Overnutrition
Y Li, CA Pollock, S Saad |
Genes & development | 2021 |
|
Epigenetic Changes Induced by Maternal Factors during Fetal Life: Implication for Type 1 Diabetes
I Barchetta, J Arvastsson, L Sarmiento, CM Cilio |
Genes & development | 2021 |
|
Identification of Diagnostic CpG Signatures in Patients with Gestational Diabetes Mellitus via Epigenome-Wide Association Study Integrated with Machine Learning
Y Liu, H Geng, B Duan, X Yang, A Ma, X Ding, S Tan |
BioMed Research International | 2021 |
|
Epigenetic Changes in Gestational Diabetes Mellitus
DF Dłuski, E Wolińska, M Skrzypczak |
International journal of molecular sciences | 2021 |
|
Effects of Environmental Conditions on Nephron Number: Modeling Maternal Disease and Epigenetic Regulation in Renal Development
L Fuhrmann, S Lindner, AT Hauser, C Höse, O Kretz, CD Cohen, MT Lindenmeyer, W Sippl, M Jung, TB Huber, N Wanner |
International journal of molecular sciences | 2021 |
|
Persistent Aortic Stiffness and Left Ventricular Hypertrophy in Children of Diabetic Mothers
V Do, L Eckersley, L Lin, ST Davidge, MK Stickland, T Ojala, J Serrano-Lomelin, LK Hornberger |
2021 | |
|
Comprehensive analysis of DNA methylation and gene expression profiles in gestational diabetes mellitus
J He, K Liu, X Hou, J Lu |
Medicine | 2021 |
|
Lifestyle Intervention in Pregnant Women With Obesity Impacts Cord Blood DNA Methylation, Which Associates With Body Composition in the Offspring
J Jönsson, KM Renault, S García-Calzón, A Perfilyev, AC Estampador, K Nørgaard, MV Lind, A Vaag, L Hjort, KF Michaelsen, EM Carlsen, PW Franks, C Ling |
Diabetes | 2021 |
|
The Transition From Undernutrition to Overnutrition Under Adverse Environments and Poverty: The Risk for Chronic Diseases
PC Leocádio, SC Lopes, RP Dias, JI Alvarez-Leite, RL Guerrant, JO Malva, RB Oriá |
Frontiers in Nutrition | 2021 |
|
Genome-wide analysis of DNA methylation and risk of cardiovascular disease in a Chinese population
Y Gao, H Pang, B Chen, CQ Wu, Y Wang, L Hou, S Wang, D Sun, X Zheng |
BMC Cardiovascular Disorders | 2021 |
|
The Placenta as a Target of Epigenetic Alterations in Women with Gestational Diabetes Mellitus and Potential Implications for the Offspring
D Lizárraga, A García-Gasca |
Epigenomes | 2021 |
|
Oxidative Stress Profile of Mothers and Their Offspring after Maternal Consumption of High-Fat Diet in Rodents: A Systematic Review and Meta-Analysis
RQ Moraes-Souza, G Vesentini, VG Paula, YK Sinzato, TS Soares, RB Gelaleti, GT Volpato, DC Damasceno, G Saretzki |
Oxidative Medicine & Cellular Longevity | 2021 |
|
Developmental Effects of (Pre-)Gestational Diabetes on Offspring: Systematic Screening Using Omics Approaches
B Shashikadze, F Flenkenthaler, JB Stöckl, L Valla, S Renner, E Kemter, E Wolf, T Fröhlich |
Genes & development | 2021 |
|
Epigenome-Wide Association Study Reveals Methylation Loci Associated With Offspring Gestational Diabetes Mellitus Exposure and Maternal Methylome
M Canouil, A Khamis, E Keikkala, S Hummel, S Lobbens, A Bonnefond, F Delahaye, E Tzala, S Mustaniemi, M Vääräsmäki, M Jarvelin, S Sebert, E Kajantie, P Froguel, T Andrew |
Diabetes care | 2021 |
|
Dichotomy in the Impact of Elevated Maternal Glucose Levels on Neonatal Epigenome
I Lim, X Lin, A Teh, Y Wu, L Chen, M He, S Chan, J MacIsaac, J Chan, K Tan, M Chong, M Kobor, K Godfrey, M Meaney, Y Lee, J Eriksson, P Gluckman, Y Chong, N Karnani |
The Journal of clinical endocrinology and metabolism | 2021 |
|
Cell-free DNA Methylation and Transcriptomic Signature Prediction of Pregnancies with Adverse Outcomes
GD Vecchio, Q Li, W Li, S Thamotharan, A Tosevska, M Morselli, K Sung, C Janzen, X Zhou, M Pellegrini, SU Devaskar |
Epigenetics : official journal of the DNA Methylation Society | 2020 |
|
Exposure to Gestational Diabetes Enriches Immune-Related Pathways in the Transcriptome and Methylome of Human Amniocytes
SE Pinney, A Joshi, V Yin, SW Min, C Rashid, DE Condon, PZ Wang |
The Journal of clinical endocrinology and metabolism | 2020 |
|
Maternal atopy and offspring epigenome-wide methylation signature
D Hanna, G Artur, D Anna, M Grzegorz, S Tomasz, M Anna, J Igor, DC Anna, H Lidia, B Andrzej |
Epigenetics : official journal of the DNA Methylation Society | 2020 |
|
Maternal dysglycaemia, changes in the infant’s epigenome modified with a diet and physical activity intervention in pregnancy: Secondary analysis of a randomised control trial
E Antoun, NT Kitaba, P Titcombe, KV Dalrymple, ES Garratt, SJ Barton, R Murray, PT Seed, JD Holbrook, MS Kobor, DT Lin, JL MacIsaac, GC Burdge, SL White, L Poston, KM Godfrey, KA Lillycrop, RC Ma |
PLoS Medicine | 2020 |
|
Epigenetic Patterns in Five-Year-Old Children Exposed to a Low Glycemic Index Dietary Intervention during Pregnancy: Results from the ROLO Kids Study
AA Geraghty, A Sexton-Oates, EC OBrien, R Saffery, FM McAuliffe |
Nutrients | 2020 |
|
Genetics and Epigenetics: New Insight on Gestational Diabetes Mellitus
MG Dalfrà, S Burlina, GG Vescovo, A Lapolla |
Frontiers in Endocrinology | 2020 |
|
Maternal diabetes and obesity influence the fetal epigenome in a largely Hispanic population
HE Rizzo, EN Escaname, NB Alana, E Lavender, J Gelfond, R Fernandez, MA Hibbs, JM King, NR Carr, CL Blanco |
Clinical Epigenetics | 2020 |
|
Effect of maternal preconceptional and pregnancy micronutrient interventions on children's DNA methylation: Findings from the EMPHASIS study
A Saffari, S Shrestha, P Issarapu, S Sajjadi, M Betts, SA Sahariah, AS Tomar, P James, A Dedaniya, DK Yadav, K Kumaran, AM Prentice, KA Lillycrop, CH Fall, GR Chandak, MJ Silver, A Saffari, S Shrestha, P Issarapu, S Sajjadi, M Betts, SA Sahariah, AS Tomar, P James, A Dedaniya, DK Yadav, K Kumaran, AM Prentice, KA Lillycrop, CH Fall, GR Chandak, MJ Silver, C Cooper, CD Gravio, SH Kehoe, RD Potdar, CS Yajnik, SS Nongmaithem, H Chopra, M Gandhi, L Kaur, M Ngum, MK Darboe, GR Goldberg, L Acolatse, S Owens, A Prentice, KA Ward, LM Jarjou, R Janha, A Saffari, S Shrestha, P Issarapu, S Sajjadi, M Betts, SA Sahariah, AS Tomar, P James, A Dedaniya, DK Yadav, K Kumaran, AM Prentice, KA Lillycrop, CH Fall, GR Chandak, MJ Silver, C Cooper, CD Gravio, SH Kehoe, RD Potdar, CS Yajnik, SS Nongmaithem, H Chopra, M Gandhi, L Kaur, M Ngum, MK Darboe, GR Goldberg, L Acolatse, S Owens, A Prentice, KA Ward, LM Jarjou, R Janha |
The American journal of clinical nutrition | 2020 |
|
Offspring susceptibility to metabolic alterations due to maternal high-fat diet and the impact of inhaled ozone used as a stressor
SJ Snow, K Broniowska, ED Karoly, AR Henriquez, PM Phillips, AD Ledbetter, MC Schladweiler, CN Miller, CJ Gordon, UP Kodavanti |
Scientific Reports | 2020 |
|
Genes and Diet in the Prevention of Chronic Diseases in Future Generations
M Franzago, D Santurbano, E Vitacolonna, L Stuppia |
International journal of molecular sciences | 2020 |
|
Gestational Diabetes Mellitus Affects Offspring’s Epigenome. Is There a Way to Reduce the Negative Consequences?
M Słupecka-Ziemilska, P Wychowański, M Puzianowska-Kuznicka |
Nutrients | 2020 |
|
Early-life nutrition and metabolic disorders in later life: a new perspective on energy metabolism
LY Zhou, MQ Deng, Q Zhang, XH Xiao |
Chinese Medical Journal | 2020 |
|
Prenatal gestational diabetes mellitus exposure and accelerated offspring DNA methylation age in early childhood
S Shiau, L Wang, H Liu, Y Zheng, A Drong, BT Joyce, J Wang, W Li, J Leng, Y Shen, R Gao, G Hu, L Hou, AA Baccarelli |
Epigenetics : official journal of the DNA Methylation Society | 2020 |
|
Analysis of potential hub genes involved in the pathogenesis of Chinese type 1 diabetic patients
S Yang, C Cao, Z Xie, Z Zhou |
Annals of translational medicine | 2020 |
|
Epigenetics in Human Obesity and Type 2 Diabetes
C Ling, T Rönn |
Cell Metabolism | 2019 |
|
Intrauterine programming of obesity and type 2 diabetes
DS Fernandez-Twinn, L Hjort, B Novakovic, SE Ozanne, R Saffery |
Diabetologia | 2019 |
|
Epigenetic Programming and Fetal Metabolic Programming
Z Zhu, F Cao, X Li |
Frontiers in Endocrinology | 2019 |
|
Determinants of Maternal Insulin Resistance during Pregnancy: An Updated Overview
U Kampmann, S Knorr, J Fuglsang, P Ovesen |
Journal of Diabetes Research | 2019 |
|
Altered Genome-Wide DNA Methylation in Peripheral Blood of South African Women with Gestational Diabetes Mellitus
S Dias, S Adam, P Rheeder, J Louw, C Pheiffer |
International journal of molecular sciences | 2019 |
