Identification and characterization of a supraclavicular brown adipose tissue in mice
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
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Research Article Development Endocrinology

Identification and characterization of a supraclavicular brown adipose tissue in mice

Abstract

A fundamental challenge to our understanding of brown adipose tissue (BAT) is the lack of an animal model that faithfully represents human BAT. Such a model is essential for direct assessment of the function and therapeutic potential of BAT depots in humans. In human adults, most of the thermoactive BAT depots are located in the supraclavicular region of the neck, while mouse studies focus on depots located in the interscapular region of the torso. We recently discovered BAT depots that are located in a region analogous to that of human supraclavicular BAT (scBAT). Here, we report that the mouse scBAT depot has morphological characteristics of classical BAT, possesses the potential for high thermogenic activity, and expresses a gene signature that is similar to that of human scBAT. Taken together, our studies reveal a mouse BAT depot that represents human BAT and provides a unique tool for developing new translatable approaches for utilizing human scBAT.

Authors

Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen

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Figure 6

Genome-wide expression analysis identifies genes specifically expressed in scBAT.

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Genome-wide expression analysis identifies genes specifically expressed ...
(A) Relative mRNA expression (qRT-PCR) of selective iBAT, WAT, or beige adipocyte markers in iBAT, scBAT, iWAT, and eWAT isolated from 8-week-old mice. Data are presented as mean ± SEM. n = 5. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; ns, nonsignificant (P > 0.05). One-way ANOVA. (B) Schematic showing the anatomical locations of iBAT, scBAT, iWAT, eWAT used for RNA-sequencing analyses. tr, trachea; cb, clavicle bone. (C) Clustered heat map of selected genes from the RNA-sequencing data in iBAT, scBAT, iWAT, and eWAT (n = 3 per tissue type). (D) Principal component analysis (PCA) of the RNA-sequencing samples. Samples were plotted in the first (PC1) and second (PC2) principal components. (E) Venn diagram showing the number of genes found to be specifically expressed in scBAT by RNA-sequencing. Sixty-one genes were significantly upregulated at least 5-fold both in scBAT compared with iBAT and in BAT compared with WAT. FC, fold change.