Identification and characterization of a supraclavicular brown adipose tissue in mice
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen
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Research Article Development Endocrinology

Identification and characterization of a supraclavicular brown adipose tissue in mice

Abstract

A fundamental challenge to our understanding of brown adipose tissue (BAT) is the lack of an animal model that faithfully represents human BAT. Such a model is essential for direct assessment of the function and therapeutic potential of BAT depots in humans. In human adults, most of the thermoactive BAT depots are located in the supraclavicular region of the neck, while mouse studies focus on depots located in the interscapular region of the torso. We recently discovered BAT depots that are located in a region analogous to that of human supraclavicular BAT (scBAT). Here, we report that the mouse scBAT depot has morphological characteristics of classical BAT, possesses the potential for high thermogenic activity, and expresses a gene signature that is similar to that of human scBAT. Taken together, our studies reveal a mouse BAT depot that represents human BAT and provides a unique tool for developing new translatable approaches for utilizing human scBAT.

Authors

Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen

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Figure 5

scBAT transplantation improves glucose tolerance, insulin sensitivity, and thermoregulation.

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scBAT transplantation improves glucose tolerance, insulin sensitivity, a...
In vivo metabolic and thermogenic analyses of iBAT and scBAT were performed on 12-week-old male mice receiving transplants of 0.1 g iBAT, 0.1 g scBAT, or no BAT (sham-operated). (A) Glucose tolerance test (GTT) area under the curve (AUC). GTTs were performed every 2 weeks after transplantation. Data are presented as mean ± SEM. n = 4–6 per group. ***P < 0.001, *P < 0.05 between BAT transplanted group and control group. (B) GTT curves. GTTs were performed at 12 weeks after transplantation. n = 4–6 per group. **P < 0.01, *P < 0.05. (C) Insulin tolerance test (ITT) curves. ITTs were performed at 12 weeks after transplantation. n = 4–6 per group. ***P < 0.001. (D) Body temperature in mice exposed to cold (4°C) for the indicated times. Short-term cold exposure experiments were performed on iBAT- and scBAT-transplanted mice at 12 weeks after transplantation. n = 3–6 per group. *P < 0.05 between BAT-transplanted group and control group. One-way ANOVA.