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Identification and characterization of a supraclavicular brown adipose tissue in mice
Qianxing Mo, … , Kristin I. Stanford, Miao-Hsueh Chen
Qianxing Mo, … , Kristin I. Stanford, Miao-Hsueh Chen
Published June 2, 2017
Citation Information: JCI Insight. 2017;2(11):e93166. https://doi.org/10.1172/jci.insight.93166.
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Research Article Development Endocrinology

Identification and characterization of a supraclavicular brown adipose tissue in mice

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Abstract

A fundamental challenge to our understanding of brown adipose tissue (BAT) is the lack of an animal model that faithfully represents human BAT. Such a model is essential for direct assessment of the function and therapeutic potential of BAT depots in humans. In human adults, most of the thermoactive BAT depots are located in the supraclavicular region of the neck, while mouse studies focus on depots located in the interscapular region of the torso. We recently discovered BAT depots that are located in a region analogous to that of human supraclavicular BAT (scBAT). Here, we report that the mouse scBAT depot has morphological characteristics of classical BAT, possesses the potential for high thermogenic activity, and expresses a gene signature that is similar to that of human scBAT. Taken together, our studies reveal a mouse BAT depot that represents human BAT and provides a unique tool for developing new translatable approaches for utilizing human scBAT.

Authors

Qianxing Mo, Jordan Salley, Tony Roshan, Lisa A. Baer, Francis J. May, Eric J. Jaehnig, Adam C. Lehnig, Xin Guo, Qiang Tong, Alli M. Nuotio-Antar, Farnaz Shamsi, Yu-Hua Tseng, Kristin I. Stanford, Miao-Hsueh Chen

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Figure 1

Identification of a supraclavicular BAT (scBAT) depot that forms during development in mice.

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Identification of a supraclavicular BAT (scBAT) depot that forms during ...
(A) Diagram of a E18.5 mouse embryo showing the positions of supraclavicular (scBAT), cervical (cBAT), interscapular (iBAT), and subscapular (sBAT) BAT depots. A, anterior; P, posterior; V, ventral; D, dorsal. Dotted lines indicate the planes of sections presented in B. (B) Representative H&E-stained histological sections (transverse sections) from E18.5 mouse embryo neck and thoracic regions showing scBAT, cBAT, iBAT, and part of sBAT depots. Scale bars: 200 μm. v, vertebrate; es, esophagus; thy, thyroid; tr, trachea; scm, sternocleidomastoid; m, muscle; nt, neural tube. *External and internal jugular veins. **Carotid artery. (C) Relative mRNA expression of BAT markers in iBAT and scBAT isolated from E18.5 mouse embryos. Data are presented as mean ± SEM. n = 6. ns, nonsignificant (P > 0.05). Two-tailed t test. (D) Western blots of BAT selective markers in iBAT and scBAT isolated from E18.5 mouse embryos. n = 3. β-Actin was used as loading control.

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