Arrestin domain containing 3 promotes Helicobacter pylori–associated gastritis by regulating protease-activated receptor 1
Yu-gang Liu, … , Quan-ming Zou, Yuan Zhuang
Yu-gang Liu, … , Quan-ming Zou, Yuan Zhuang
Published July 7, 2020
Citation Information: JCI Insight. 2020;5(15):e135849. https://doi.org/10.1172/jci.insight.135849.
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Research Article Gastroenterology Infectious disease

Arrestin domain containing 3 promotes Helicobacter pylori–associated gastritis by regulating protease-activated receptor 1

Abstract

Arrestin domain containing 3 (ARRDC3) represents a newly discovered α-arrestin involved in obesity, inflammation, and cancer. Here, we demonstrate a proinflammation role of ARRDC3 in Helicobacter pylori–associated gastritis. Increased ARRDC3 was detected in gastric mucosa of patients and mice infected with H. pylori. ARRDC3 in gastric epithelial cells (GECs) was induced by H. pylori, regulated by ERK and PI3K-AKT pathways in a cagA-dependent manner. Human gastric ARRDC3 correlated with the severity of gastritis, and mouse ARRDC3 from non-BM–derived cells promoted gastric inflammation. This inflammation was characterized by the CXCR2-dependent influx of CD45+CD11b+Ly6C–Ly6G+ neutrophils, whose migration was induced via the ARRDC3-dependent production of CXCL2 by GECs. Importantly, gastric inflammation was attenuated in Arrdc3–/– mice but increased in protease-activated receptor 1–/– (Par1–/–) mice. Mechanistically, ARRDC3 in GECs directly interacted with PAR1 and negatively regulated PAR1 via ARRDC3-mediated lysosomal degradation, which abrogated the suppression of CXCL2 production and following neutrophil chemotaxis by PAR1, thereby contributing to the development of H. pylori–associated gastritis. This study identifies a regulatory network involving H. pylori, GECs, ARRDC3, PAR1, and neutrophils, which collectively exert a proinflammatory effect within the gastric microenvironment. Efforts to inhibit this ARRDC3-dependent pathway may provide valuable strategies in treating of H. pylori–associated gastritis.

Authors

Yu-gang Liu, Yong-sheng Teng, Zhi-guo Shan, Ping Cheng, Chuan-jie Hao, Yi-pin Lv, Fang-yuan Mao, Shi-ming Yang, Weisan Chen, Yong-liang Zhao, Nan You, Quan-ming Zou, Yuan Zhuang

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Figure 4

ARRDC3 has proinflammatory effects during H. pylori infection.

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ARRDC3 has proinflammatory effects during H. pylori infection. (A and B)...
(A and B) Histological scores of inflammation and IL-6 and TNF-α protein in gastric mucosa of WT H. pylori–infected WT and Arrdc3–/– mice (n = 6) (A) or in gastric mucosa of WT H. pylori–infected BM chimera mice (n = 5) (B) on day 28 p.i. were compared. (C and D) Representative H&E staining images showed inflammation in gastric antra of WT H. pylori–infected WT and Arrdc3–/– mice (C) or in gastric antra of WT H. pylori–infected BM chimera mice (D) on day 28 p.i. Scale bars: 100 μm. (E and F) ARRDC3 expression (E) and ARRDC3 protein (F) in gastric mucosa of H. pylori–infected patients with mild (n = 17), moderate (n = 17), severe inflammation (n = 17), and uninfected donors with normal gastric histopathology (n = 19) was compared. (G) CD45+CD11b+Ly6C–Ly6G+ neutrophil levels in gastric mucosa of WT H. pylori–infected WT and Arrdc3–/– mice (n = 6) or in gastric mucosa of WT H. pylori–infected BM chimera mice (n = 5) on day 28 p.i. were compared. (H) Histological scores of inflammation in gastric mucosa of WT H. pylori–infected mice injected with Abs against Ly6G or corresponding isotype control Abs on day 28 p.i. were compared (n = 5). Scale bars: 100 μm. Data are representative of 2 independent experiments. Data are mean ± SEM and analyzed by Student t test, Mann-Whitney U test, and 1-way ANOVA. Western blot results are run in parallel and contemporaneously. *P < 0.05, **P < 0.01 for groups connected by horizontal lines.