Go to The Journal of Clinical Investigation
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Immunology
    • Metabolism
    • Nephrology
    • Oncology
    • Pulmonology
    • All ...
  • Videos
  • Collections
    • Resource and Technical Advances
    • Clinical Medicine
    • Reviews
    • Editorials
    • Perspectives
    • Top read articles
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • In-Press Preview
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
Circulating microRNA biomarkers for metastatic disease in neuroblastoma patients
Fjoralba Zeka, … , Pieter Mestdagh, Jo Vandesompele
Fjoralba Zeka, … , Pieter Mestdagh, Jo Vandesompele
Published December 6, 2018
Citation Information: JCI Insight. 2018;3(23):e97021. https://doi.org/10.1172/jci.insight.97021.
View: Text | PDF
Research Article Genetics Oncology

Circulating microRNA biomarkers for metastatic disease in neuroblastoma patients

  • Text
  • PDF
Abstract

In this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of noninvasive biomarkers with potential in monitoring metastatic disease. After determining the circulating neuroblastoma miRNome, 743 miRNAs were screened in 2 independent cohorts of 131 and 54 patients. Evaluation of serum miRNA variance in a model testing for tumor stage, MYCN status, age at diagnosis, and overall survival revealed tumor stage as the most significant factor impacting miRNA abundance in neuroblastoma serum. Differential abundance analysis between patients with metastatic and localized disease revealed 9 miRNAs strongly associated with metastatic stage 4 disease in both patient cohorts. Increasing levels of these miRNAs were also observed in serum from xenografted mice bearing human neuroblastoma tumors. Moreover, murine serum miRNA levels were strongly associated with tumor volume. These findings were validated in longitudinal serum samples from metastatic neuroblastoma patients, where the 9 miRNAs were associated with disease burden and treatment response.

Authors

Fjoralba Zeka, Anneleen Decock, Alan Van Goethem, Katrien Vanderheyden, Fleur Demuynck, Tim Lammens, Hetty H. Helsmoortel, Joëlle Vermeulen, Rosa Noguera, Ana P. Berbegall, Valérie Combaret, Gudrun Schleiermacher, Geneviève Laureys, Alexander Schramm, Johannes H. Schulte, Sven Rahmann, Julie Bienertová-Vašků, Pavel Mazánek, Marta Jeison, Shifra Ash, Michael D. Hogarty, Mirthala Moreno-Smith, Eveline Barbieri, Jason Shohet, Frank Berthold, Tom Van Maerken, Frank Speleman, Matthias Fischer, Katleen De Preter, Pieter Mestdagh, Jo Vandesompele

×

Figure 8

Serum abundance of disease burden miRNA markers changes during treatment of stage 4 neuroblastoma patients.

Options: View larger image (or click on image) Download as PowerPoint
Serum abundance of disease burden miRNA markers changes during treatment...
(A) The disease course for each of the patients (P1–P5) is depicted, including the time points of serum collection and occurrence of disease events (relapse, progressive disease, and death). (B) For each of the individual patients, the change in average abundance level of the 9 disease burden miRNA markers in serum is shown. Note that at t1, P1, P3, and P5 are considered treatment responders, while P2 and P4 are considered nonresponders.

Copyright © 2023 American Society for Clinical Investigation
ISSN 2379-3708

Sign up for email alerts