Heat maps comparing cellular marker positivity by denoting cells as either positive or negative for each marker during mock, dengue virus 2 (DENV-2), and DENV-4 infections. For all plots, markers are ordered vertically by hierarchical clustering of the rows, which places markers with similar expression profiles closer together. Statistical significance was determined using a 2-sided Fisher’s exact test at a threshold of α = 10^–5 with Bonferroni correction for multiple testing. Cellular markers not representing a significant difference between infection conditions at this threshold are labeled with “NS”; all others are significant. Calculations were made using the event counts from mass cytometry by time-of-flight (CyTOF) data, as determined by autogating of cells positive for each marker based on the 99th percentile threshold, which was set for mock treatment at 8 hours after infection (hpi). The comparative effects on each marker were calculated as the log₁₀ of the relative risk for positive marker frequency between the indicated infection conditions. The histograms within the heat map color scales show the total number of cells matching that intensity range, revealing the overall distribution of the values. One representative DC donor of seven is shown. (A) DENV-2 infection and mock treatment are compared. Red indicates greater marker expression during DENV-2 infection, and blue indicates greater marker expression during mock treatment. (B) DENV-4 infection and mock treatment are compared. Red indicates greater marker expression during DENV-4 infection, and blue indicates greater marker expression during mock treatment. (C) DENV-4 and DENV-2 infections are compared. Red indicates greater marker expression during DENV-4 infection, and blue indicates greater marker expression during DENV-2 infection. White indicates no difference in marker expression between the infection conditions.