Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct
Julie G. Burel, … , James S. McCarthy, Denise L. Doolan
Julie G. Burel, … , James S. McCarthy, Denise L. Doolan
Published February 9, 2017
Citation Information: JCI Insight. 2017;2(3):e87499. https://doi.org/10.1172/jci.insight.87499.
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Research Article Cell biology

Polyfunctional and IFN-γ monofunctional human CD4+ T cell populations are molecularly distinct

Abstract

Pathogen-specific polyfunctional T cell responses have been associated with favorable clinical outcomes, but it is not known whether molecular differences exist between polyfunctional and monofunctional cytokine-producing T cells. Here, we report that polyfunctional CD4+ T cells induced during Plasmodium falciparum (P. falciparum) blood-stage infection in humans have a unique transcriptomic profile compared with IFN-γ monofunctional CD4+ T cells and, thus, are molecularly distinct. The 14-gene signature revealed in P. falciparum–reactive polyfunctional T cells is associated with cytokine signaling and lymphocyte chemotaxis, and systems biology analysis identified IL-27 as an upstream regulator of the polyfunctional gene signature. Importantly, the polyfunctional gene signature is largely conserved in Influenza-reactive polyfunctional CD4+ T cells, suggesting that polyfunctional T cells have core characteristics independent of pathogen specificity. This study provides the first evidence to our knowledge that consistent molecular differences exist between polyfunctional and monofunctional CD4+ T cells.

Authors

Julie G. Burel, Simon H. Apte, Penny L. Groves, James S. McCarthy, Denise L. Doolan

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