(A) Schematic representation of the Clec4g-CreERT2⁺ construct and ZsGreen-Ai6 reporter system. (B) Representative confocal image of a portal triad depicting a portal vein (PV), hepatic artery (HA), bile duct (BD), liver sinusoidal endothelial cells (LSECs), and lymphatic vessel (LV) 1 month after tamoxifen administration. Scale bars: 200 μm; 50 μm in insets. (C) Representative analysis of flow cytometry of CD45^–CD31⁺ liver endothelial cells (LECs) showing ZsGreen⁺ and ZsGreen^– fractions, 7 days after tamoxifen administration (Top). Quantification of ZsGreen⁺ cells among CD45^–CD31⁺ liver endothelial cells and CD45⁺CD31^– immune cells by flow cytometry (bottom; paired t test); n = 4 mice. (D) Representative confocal images at 7 days, 1 month, and 3 months after tamoxifen administration at weaning age. Scale bars: 200 μm; 100 μm in insets. PVs (red arrows) and CVs (yellow arrows). (E) Quantification of ZsGreen⁺ area in CVs and PVs over time (Kruskal-Wallis test); n = 4 mice per time point. (F) Quantification of ZsGreen⁺ area in PVs stratified by size (small, intermediate, large) at the indicated time points (2-way ANOVA); n = 4 mice per time point. (G) Representative confocal images of large PVs (red arrows) branching into terminal venules (white arrows), showing progressive accumulation of Clec4g⁺ZsGreen⁺ cells over time. Scale bars: 200 μm. (H) Quantification of DAPI⁺ nuclei within ZsGreen⁺ regions of CVs and PVs over time (Kruskal-Wallis test). SMA, smooth muscle actin; Lyve1, lymphatic vessel endothelial hyaluronan receptor 1. Scatter dot plot showing individual values; data are shown as mean ± SD. Box-and-whisker plots, with whiskers indicating minimum/maximum, boxes indicating 25th to 75th percentile, and horizontal line indicating the median. ***P<0.001, **P<0.01, *P<0.05.