Proximal ganglionic intestine in Hirschsprung disease is fibrotic and stiff

Prisca C. Obidike; Britney A. Hsu; Chioma Moneme; Oluyinka O. Olutoye II; Walker D. Short; Mary Hui Li; Swathi Balaji; Yuwen Zhang; Sundeep G. Keswani; Lily S. Cheng

doi:10.1172/jci.insight.199820

Proximal ganglionic intestine in Hirschsprung disease is fibrotic and stiff

Prisca C. Obidike,¹ Britney A. Hsu,² Chioma Moneme,¹ Oluyinka O. Olutoye II,² Walker D. Short,² Mary Hui Li,² Swathi Balaji,² Yuwen Zhang,¹ Sundeep G. Keswani,³ and Lily S. Cheng¹

Published February 10, 2026 - More info

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Abstract

Hirschsprung disease (HSCR) is a congenital intestinal disorder characterized by the absence of ganglia in the distal intestine. Despite surgical resection of the aganglionic intestine and pull-through surgery, HSCR patients still experience bowel dysfunction, indicating that latent abnormalities may also exist in the proximal ganglionic intestine. To elucidate possible causes of postoperative bowel dysfunction in HSCR, we investigated differences in the proximal ganglionic intestine using an animal model of HSCR (Ednrb-null mice) and validated our findings in tissue from human HSCR patients. We found that the proximal ganglionic colon of HSCR mice exhibited greater stiffness and fibrosis than their wild-type littermates. Similarly, submucosal fibrosis was significantly greater in the proximal ganglionic intestine of HSCR patients than in intestinal tissue from age and site-matched controls. Furthermore, we observed dysregulated expression of extracellular matrix (ECM)-related genes in the proximal ganglionic intestine of HSCR mice compared to controls. We conclude that increased fibrosis, stiffness, and alterations in ECM composition may contribute to persistent dysfunction of the ganglionic intestine in HSCR. These findings add to the growing body of literature that describe abnormalities in the proximal ganglionic intestine of HSCR and suggest that HSCR is not limited to the aganglionic intestine alone.

Proximal ganglionic intestine in Hirschsprung disease is fibrotic and stiff

Prisca C. Obidike,¹ Britney A. Hsu,² Chioma Moneme,¹ Oluyinka O. Olutoye II,² Walker D. Short,² Mary Hui Li,² Swathi Balaji,² Yuwen Zhang,¹ Sundeep G. Keswani,³ and Lily S. Cheng¹

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Proximal ganglionic intestine in Hirschsprung disease is fibrotic and stiff

Prisca C. Obidike,1 Britney A. Hsu,2 Chioma Moneme,1 Oluyinka O. Olutoye II,2 Walker D. Short,2 Mary Hui Li,2 Swathi Balaji,2 Yuwen Zhang,1 Sundeep G. Keswani,3 and Lily S. Cheng1

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Prisca C. Obidike,¹ Britney A. Hsu,² Chioma Moneme,¹ Oluyinka O. Olutoye II,² Walker D. Short,² Mary Hui Li,² Swathi Balaji,² Yuwen Zhang,¹ Sundeep G. Keswani,³ and Lily S. Cheng¹