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ResearchIn-Press PreviewImmunologyOncology Open Access | 10.1172/jci.insight.190149

Photon and particle radiotherapy induce redundant modular chemotaxis of human lymphocytes

Joscha A. Kraske,1 Michael M. Allers,2 Aleksei Smirnov,2 Bénédicte Lenoir,3 Azaz Ahmed,1 Meggy Suarez-Carmona,4 Mareike Hampel,3 Damir Krunic,5 Alexandra Tietz-Dalfuß,2 Tizian Beikert,2 Jonathan M. Schneeweiss,2 Stephan Brons,6 Dorothee Albrecht,2 Thuy Trinh,2 Muzi Liu,2 Nathalia A. Giese,7 Christin Glowa,2 Jakob Liermann,2 Ramon Lopez Perez,2 Dirk Jäger,1 Jürgen Debus,2 Niels Halama,1 Peter E. Huber,2 and Thomas Walle1

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Kraske, J. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Allers, M. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Smirnov, A. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Lenoir, B. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Ahmed, A. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Suarez-Carmona, M. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Hampel, M. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Krunic, D. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Tietz-Dalfuß, A. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Beikert, T. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Schneeweiss, J. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Brons, S. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Albrecht, D. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Trinh, T. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Liu, M. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Giese, N. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Glowa, C. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Liermann, J. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Lopez Perez, R. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Jäger, D. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Debus, J. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Halama, N. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Huber, P. in: PubMed | Google Scholar

1Department of Medical Oncology, National Center for Tumor Diseases (NCT) & Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

2Department of Radiation Oncology, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

3Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany

4Division of Translational Immunotherapy, German Cancer Research Center (DKFZ), Heidelberg, Germany

5Light Microscopy Facility, German Cancer Research Center (DKFZ), Heidelberg, Germany

6Heidelberg Ion Therapy Center (HIT), Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany

7Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany

Find articles by Walle, T. in: PubMed | Google Scholar

Published August 14, 2025 - More info

JCI Insight. https://doi.org/10.1172/jci.insight.190149.
Copyright © 2025, Kraske et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published August 14, 2025 - Version history
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Abstract

Radiotherapy triggers chemokine release and leukocyte infiltration in pre-clinical models through activation of the senescence-associated secretory phenotype (SASP). However, effects of irradiation on senescence and SASP in human tissue and in the context of particle radiotherapy remain unclear. Here, we analyzed chemokine patterns after radiotherapy of human pancreatic tumors and cancer cell lines. We show that irradiated tumor cells co-express SASP chemokines in defined modules. These chemokine modules correlated with infiltration of distinct leukocyte subtypes expressing cognate receptors. We developed a patient-derived pancreatic tumor explant system, which confirmed our identified radiation-induced chemokine modules. Chemokine modules were partially conserved in cancer cells in response to photon and particle irradiation showing a dose-dependent plateau effect and induced subsequent migration of NK and T cell populations. Hence, our work reveals redundant interactions of cancer cells and immune cells in human tissue, suggesting that targeting multiple chemokines is required to efficiently perturb leukocyte infiltration after photon or particle radiotherapy.

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Version history
  • Version 1 (August 14, 2025): In-Press Preview
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