(A) Representative hematoxylin and eosin–stained pancreas sections. Scale bar: 50 μm. (B) Quantitation of acinar cell loss by histology scoring of pancreas sections. Individual values with the mean and standard deviation are shown. The number of sections evaluated, from left to right, were 10, 14, 25, 9, 10, 23, 18, 9, 10, 24, 20, 8, 8, 6, 7, and 8. Relative to C57BL/6N controls, the area of normal acini was significantly smaller in T7C140S mice (P = 0.0691 at 1 month, P = 0.035 at 3 months, P < 0.0001 at 6 months, P = 0.0229 at 12 months), CPA1 N256K mice (P < 0.0001 at 1, 3, 6 months, P = 0.0006 at 12 months), and T7C140S × CPA1 N256K mice (P < 0.0001 at all ages). (C) Pancreas weight in mg units. Individual values with the mean and standard deviation are shown. The number of pancreata measured, from left to right, were 23, 10, 25, 9, 15, 17, 16, 15, 25, 10, 25, 12, 10, 13, 4, and 8. Relative to C57BL/6N controls, the pancreas weight of T7C140S, CPA1 N256K, and T7C140S × CPA1 N256K mice was significantly lower at all ages (P < 0.0001), except for T7C140S (P = 0.0159) and CPA1 N256K (P = 0.0008) at 1 month. The difference of means was analyzed by 1-way ANOVA followed by Tukey’s post hoc test.