PFKFB3 controls acinar IP3R-mediated Ca2+ overload to regulate acute pancreatitis severity
Tan Zhang, Shengchuan Chen, Liang Li, Yuepeng Jin, Siying Liu, Zhu Liu, Fengyu Shi, Lifen Xie, Panpan Guo, Andrew C. Cannon, Akmal Ergashev, Haiping Yao, Chaohao Huang, Baofu Zhang, Lijun Wu, Hongwei Sun, Siming Chen, Yunfeng Shan, Zhengping Yu, Ezequiel J. Tolosa, Jianghuai Liu, Martin E. Fernandez-Zapico, Feng Ma, Gang Chen
Tan Zhang, Shengchuan Chen, Liang Li, Yuepeng Jin, Siying Liu, Zhu Liu, Fengyu Shi, Lifen Xie, Panpan Guo, Andrew C. Cannon, Akmal Ergashev, Haiping Yao, Chaohao Huang, Baofu Zhang, Lijun Wu, Hongwei Sun, Siming Chen, Yunfeng Shan, Zhengping Yu, Ezequiel J. Tolosa, Jianghuai Liu, Martin E. Fernandez-Zapico, Feng Ma, Gang Chen
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Research Article Inflammation Metabolism

PFKFB3 controls acinar IP3R-mediated Ca2+ overload to regulate acute pancreatitis severity

Abstract

Acute pancreatitis (AP) is among the most common hospital gastrointestinal diagnoses; understanding the mechanisms underlying the severity of AP is critical for development of new treatment options for this disease. Here, we evaluate the biological function of phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) in AP pathogenesis in 2 independent genetically engineered mouse models of AP. PFKFB3 was elevated in AP and severe AP (SAP), and KO of Pfkfb3 abrogated the severity of alcoholic SAP (FAEE-SAP). Using a combination of genetic, pharmacological, and molecular studies, we defined the interaction of PFKFB3 with inositol 1,4,5-trisphosphate receptor (IP3R) as a key event mediating this phenomenon. Further analysis demonstrated that the interaction between PFKFB3 and IP3R promotes FAEE-SAP severity by altering intracellular calcium homeostasis in acinar cells. Together, our results support a PFKFB3-driven mechanism controlling AP pathobiology and define this enzyme as a therapeutic target to ameliorate the severity of this condition.

Authors

Tan Zhang, Shengchuan Chen, Liang Li, Yuepeng Jin, Siying Liu, Zhu Liu, Fengyu Shi, Lifen Xie, Panpan Guo, Andrew C. Cannon, Akmal Ergashev, Haiping Yao, Chaohao Huang, Baofu Zhang, Lijun Wu, Hongwei Sun, Siming Chen, Yunfeng Shan, Zhengping Yu, Ezequiel J. Tolosa, Jianghuai Liu, Martin E. Fernandez-Zapico, Feng Ma, Gang Chen

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Figure 1

PFKFB3 is upregulated in AP and SAP.

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PFKFB3 is upregulated in AP and SAP. (A) Pancreatic tissues were collect...
(A) Pancreatic tissues were collected 10 or 22 hours after injection of saline or caerulein (CAE), followed RNA-Seq (the National Genomics Data Center Accession no. CRA002365). Heatmap shows differentially expressed genes (DEGs) of HIF1 signaling pathway in CAE-AP model. (B–D) Western blotting and qPCR detected the PFKFB3 level in CAE-AP (B), FAEE-SAP (C) and NaTc-SAP (D) models. (E–J) IHC analysis of PFKFB3 in pancreas from control group (n = 5) and CAE-AP (E), FAEE-SAP (F), and NaTc-SAP (G) models (n = 5). Quantification of PFKFB3 intensity in 3 AP models is shown in H–J, respectively. Scale bar: 200 μm. (K and L) IHC analysis of PFKFB3 in pancreas from patients with AP or SAP (n = 5). Scale bar: 80 μm. (M) PFKFB3 levels were detected using Western blotting in AR42J cells treated with TNFA (80 ng/mL; 6 hours) and CoCl2 (200 μmol/L; 6 hours). P values calculated using unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars show mean ± SD. Each experiment was performed at least in triplicate.