Go to The Journal of Clinical Investigation
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Immunology
    • Metabolism
    • Nephrology
    • Oncology
    • Pulmonology
    • All ...
  • Videos
  • Collections
    • Resource and Technical Advances
    • Clinical Medicine
    • Reviews
    • Editorials
    • Perspectives
    • Top read articles
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • In-Press Preview
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Supplemental material
  • Version history
  • Article usage
  • Citations to this article
Advertisement

ResearchIn-Press PreviewImmunologyInfectious disease Open Access | 10.1172/jci.insight.161450

Germline IgM predicts T-cell immunity to Pneumocystis

Kristin Noell,1 Guixiang Dai,1 Dora Pungan,1 Anna Ebacher,1 Janet E. McCombs,1 Samuel J. Landry,2 and Jay K. Kolls1

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Noell, K. in: JCI | PubMed | Google Scholar

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Dai, G. in: JCI | PubMed | Google Scholar

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Pungan, D. in: JCI | PubMed | Google Scholar |

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Ebacher, A. in: JCI | PubMed | Google Scholar

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by McCombs, J. in: JCI | PubMed | Google Scholar

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Landry, S. in: JCI | PubMed | Google Scholar |

1Departments of Pediatrics & Medicine, Tulane University School of Medicine, New Orleans, United States of America

2Department of Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, United States of America

Find articles by Kolls, J. in: JCI | PubMed | Google Scholar |

Published August 2, 2022 - More info

JCI Insight. https://doi.org/10.1172/jci.insight.161450.
Copyright © 2022, Noell et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published August 2, 2022 - Version history
View PDF
Abstract

Pneumocystis is the most common fungal pulmonary infection in children under 5. In children with primary immunodeficiency, Pneumocystis often presents at 3-6 months that coincides with the nadir of maternal IgG and where IgM is the dominant immunoglobulin isotype. Since B cells are the dominant antigen-presenting cells for Pneumocystis, we hypothesized the presence of fungal specific IgMs in human and mice and that these IgM specificities would predict T cell antigens. We detected fungal specific IgMs in human and mouse serum and utilized immunoprecipitation to determine if any antigens were similar across donors. We then assessed T cell responses to these antigens. We found anti-Pneumocystis IgM in wild-type mice as well as Aicda-/- mice and in human cord blood. Immunoprecipitation of Pneumocystis murina with human cord blood identified shared antigens among these donors. Using class II MHC binding prediction, we designed peptides with these antigens and identified robust peptide specific lung T cell responses after P. murina infection. After mice were immunized with two of the antigens, adoptive transfer of vaccine elicited CD4+ T cells showed effector activity suggesting that these antigens contain protective Pneumocystis epitopes. These data support the notion that germline encoded IgM B-cell receptors are critical in antigen presentation and T cell priming in early Pneumocystis infection.

Supplemental material

View

Version history
  • Version 1 (August 2, 2022): In-Press Preview

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Supplemental material
  • Version history
Advertisement
Advertisement

Copyright © 2022 American Society for Clinical Investigation
ISSN 2379-3708

Sign up for email alerts