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Citations to this article

A conserved annexin A6–mediated membrane repair mechanism in muscle, heart, and nerve
Alexis R. Demonbreun, … , Robert Vassar, Elizabeth M. McNally
Alexis R. Demonbreun, … , Robert Vassar, Elizabeth M. McNally
Published July 22, 2022
Citation Information: JCI Insight. 2022;7(14):e158107. https://doi.org/10.1172/jci.insight.158107.
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Research Article Cardiology Muscle biology Article has an altmetric score of 13

A conserved annexin A6–mediated membrane repair mechanism in muscle, heart, and nerve

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Abstract

Membrane instability and disruption underlie myriad acute and chronic disorders. Anxa6 encodes the membrane-associated protein annexin A6 and was identified as a genetic modifier of muscle repair and muscular dystrophy. To evaluate annexin A6’s role in membrane repair in vivo, we inserted sequences encoding green fluorescent protein (GFP) into the last coding exon of Anxa6. Heterozygous Anxa6gfp mice expressed a normal pattern of annexin A6 with reduced annexin A6GFP mRNA and protein. High-resolution imaging of wounded muscle fibers showed annexin A6GFP rapidly formed a repair cap at the site of injury. Injured cardiomyocytes and neurons also displayed repair caps after wounding, highlighting annexin A6–mediated repair caps as a feature in multiple cell types. Using surface plasmon resonance, we showed recombinant annexin A6 bound phosphatidylserine-containing lipids in a Ca2+- and dose-dependent fashion with appreciable binding at approximately 50 μM Ca2+. Exogenously added recombinant annexin A6 localized to repair caps and improved muscle membrane repair capacity in a dose-dependent fashion without disrupting endogenous annexin A6 localization, indicating annexin A6 promotes repair from both intracellular and extracellular compartments. Thus, annexin A6 orchestrates repair in multiple cell types, and recombinant annexin A6 may be useful in additional chronic disorders beyond skeletal muscle myopathies.

Authors

Alexis R. Demonbreun, Elena Bogdanovic, Lauren A. Vaught, Nina L. Reiser, Katherine S. Fallon, Ashlee M. Long, Claire C. Oosterbaan, Michele Hadhazy, Patrick G.T. Page, Prem Raj B. Joseph, Gabrielle Cowen, Alexander M. Telenson, Ammaarah Khatri, Katherine R. Sadleir, Robert Vassar, Elizabeth M. McNally

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Total citations by year

Year: 2025 2024 2023 2022 Total
Citations: 3 5 4 2 14
Citation information
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Citations to this article in year 2024 (5)

Title and authors Publication Year
Physiological stress improves stem cell modeling of dystrophic cardiomyopathy
Fullenkamp DE, Willis AB, Curtin JL, Amaral AP, Dittloff KT, Harris SI, Chychula IA, Holgren CW, Burridge PW, Russell B, Demonbreun AR, McNally EM
Disease models & mechanisms 2024
Caveolae disassemble upon membrane lesioning and foster cell survival
Štefl M, Takamiya M, Middel V, Tekpınar M, Nienhaus K, Beil T, Rastegar S, Strähle U, Nienhaus GU
iScience 2024
Annexin A6 mitigates neurological deficit in ischemia/reperfusion injury by promoting synaptic plasticity.
Wang Y, Yang Z, Wang R, Zheng Y, Han Z, Fan J, Yan F, Liu P, Luo Y
CNS Neuroscience & Therapeutics 2024
The extracellular matrix differentially directs myoblast motility and differentiation in distinct forms of muscular dystrophy: Dystrophic matrices alter myoblast motility
Long AM, Kwon JM, Lee G, Reiser NL, Vaught LA, O\u2019Brien JG, Page PG, Hadhazy M, Reynolds JC, Crosbie RH, Demonbreun AR, McNally EM
Matrix biology : journal of the International Society for Matrix Biology 2024
Calcium influx rapidly establishes distinct spatial recruitments of Annexins to cell wounds
Nakamura M, Parkhurst SM
Genetics 2024

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