(A) Immunocytochemistry analysis of fibroblasts from LAM tissues of WT and Arom^hum mice that were isolated via differential plating. PDGFRα and ERα expression were observed in both WT and Arom^hum LAM fibroblasts (n = 10 mice/group in 4 technical replicates). (B) Flow cytometry histogram profiles of freshly isolated PDGFRα⁺ cells from LAM of WT and Arom^hum mice. Quadriceps muscle (QM) was used as a control (n = 3). (C) Representative flow cytometry dot plots of ERα⁺ and PDGFRα⁺ cell populations (n = 3). (D) Dot plots representing the proportions of median fluorescence intensity (MFI) of ERα and PDGFRα and the number of cells in the Q2 gate of C. Numbers indicate MFI of ERα and PDGFRα and the cell counts for the plots in C. Both QM and LAM muscles were isolated uniformly from both WT and Arom^hum mice (n = 3). (E) The total number of ERα⁺ cells from LAM tissues was quantified via flow cytometry in Arom^hum versus WT mice (n = 20 mice/group, 5 technical replicates). (F) Cell cycle stages from freshly isolated LAM cells of Arom^hum mice were analyzed via flow cytometry. DNA content of ERα⁺ and ERα^– cells was stained by FxCycle dye, and area histograms were used to quantify G1, S, and G2 phases (n = 5 mice/group). (G) Flow cytometry histogram profiles of complement protein C4b⁺ cells, indicating Mmp3^hi cluster, from LAM tissues of WT and Arom^hum mice (n = 3). (H) Fluorescence Western blots of whole LAM tissue homogenates from WT and Arom^hum. Two MMP3 bands were detected at ~45 kDa and ~27 kDa, PCNA protein was detected at ~30 kDa, and Tgfbi protein BGH3 was recognized at ~65 kDa. Quantification was performed by normalizing to total protein detected by Ponceau S staining (n = 4 mice/group). Box plots represent median with minimum and maximum values as whiskers and groups were compared using 2-sided t tests. *P < 0.05.