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ResearchIn-Press PreviewDevelopmentVascular biology Open Access | 10.1172/jci.insight.151222

Tentorial venous anatomy of mice and men

Pashayar P. Lookian,1 Vikram Chandrashekhar,2 Anthony Cappadona,1 Jean-Paul Bryant,3 Vibhu Chandrashekhar,4 Jessa M. Tunacao,5 Danielle R. Donahue,6 Jeeva P. Munasinghe,6 James G. Smirniotopoulos,7 John D. Heiss,3 Zhengping Zhuang,1 and Jared S. Rosenblum1

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Lookian, P. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Chandrashekhar, V. in: PubMed | Google Scholar

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

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1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Bryant, J. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Chandrashekhar, V. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Tunacao, J. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Donahue, D. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Munasinghe, J. in: PubMed | Google Scholar

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Smirniotopoulos, J. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Heiss, J. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Zhuang, Z. in: PubMed | Google Scholar |

1Neuro-Oncology Branch, NIH, Bethesda, United States of America

2Biomedical Engineering, Johns Hopkins University, Baltimore, United States of America

3Surgical Neurology Branch, NIH/NINDS, Bethesda, United States of America

4NeuroSimplicity, LLC, North Bethesda, United States of America

5Radiology, Stony Brook University, Stony Brook, United States of America

6Mouse Imaging Facility, NIH/NINDS, Bethesda, United States of America

7Radiology, George Washington University, Washington DC, United States of America

Find articles by Rosenblum, J. in: PubMed | Google Scholar |

Published September 21, 2021 - More info

JCI Insight. https://doi.org/10.1172/jci.insight.151222.
Copyright © 2021, Lookian et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published September 21, 2021 - Version history
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Abstract

We recently described a previously unknown trans-tentorial venous system (TTVS) connecting venous drainage throughout the brain in humans. Prior to this finding, it was believed that the embryologic tentorial plexus regresses, resulting in a largely avascular tentorium. Our finding contradicted this understanding and necessitated further investigation into the development of the newly described TTVS. Herein we sought to investigate mice as a model to study the development of this system. First, using vascular casting and ex vivo micro-computed tomography (micro-CT), we demonstrate that this TTVS is conserved in adult mice. Next, using high-resolution magnetic resonance imaging (MRI), we found the primitive tentorial venous plexus in murine embryo at day 14.5. We also found that, at this embryologic stage, the tentorial plexus drains the choroid plexus. Finally, using vascular casting and micro-CT, we found that the TTVS is the dominant venous drainage in the early postnatal period (P8). Herein, we demonstrate that the TTVS is conserved between mice and humans and present a longitudinal study of its development. In addition, our findings establish mice as a translational model for further study of this newly described system and its relationship to intracranial physiology.

Graphical Abstract
graphical abstract
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Version history
  • Version 1 (September 21, 2021): In-Press Preview
  • Version 2 (November 8, 2021): Electronic publication
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