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MYH9 facilitates autoregulation of adipose tissue depot development
Sin Ying Cheung, … , Liang Li, Brian J. Feldman
Sin Ying Cheung, … , Liang Li, Brian J. Feldman
Published May 10, 2021
Citation Information: JCI Insight. 2021;6(9):e136233. https://doi.org/10.1172/jci.insight.136233.
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Research Article Development Endocrinology

MYH9 facilitates autoregulation of adipose tissue depot development

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Abstract

White adipose tissue not only serves as a reservoir for energy storage but also secretes a variety of hormonal signals and modulates systemic metabolism. A substantial amount of adipose tissue develops in early postnatal life, providing exceptional access to the formation of this important tissue. Although a number of factors have been identified that can modulate the differentiation of progenitor cells into mature adipocytes in cell-autonomous assays, it remains unclear which are connected to physiological extracellular inputs and are most relevant to tissue formation in vivo. Here, we elucidate that mature adipocytes themselves signal to adipose depot–resident progenitor cells to direct depot formation in early postnatal life and gate adipogenesis when the tissue matures. Our studies revealed that as the adipose depot matures, a signal generated in mature adipocytes is produced, converges on progenitor cells to regulate the cytoskeletal protein MYH9, and attenuates the rate of adipogenesis in vivo.

Authors

Sin Ying Cheung, Mohd Sayeed, Krishnamurthy Nakuluri, Liang Li, Brian J. Feldman

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Figure 2

ADAMTS1 regulates MYH9 in APCs.

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ADAMTS1 regulates MYH9 in APCs.
(A) RT-qPCR quantifying Myh9 expression ...
(A) RT-qPCR quantifying Myh9 expression levels in APCs isolated from subcutaneous white adipose tissue depots from 3-week-old male wild-type and Adamts1-transgenic (Adamts1Tg) mice (n = 3). (B) Images of immunocytochemistry showing the levels and localization of MYH9 and F-actin (phalloidin) in APCs isolated from 3-week-old male wild-type mice compared with Adamts1Tg littermate mice. Original magnification, ×400. (C) RT-qPCR quantifying Myh9 expression levels in APCs isolated from wild-type mice and treated with recombinant ADAMTS1 (rADAMTS1) (100 ng/mL), rADAMTS1, and IWP-2 (2 μM/mL) (n = 3). (D) Immunoblots (left) with quantification (right) of MYH9 levels in APCs from wild-type mice treated with rADAMTS1 (100 ng/mL), IWP-2 (2 μM/mL), or both rADAMTS1 and IWP-2 (n = 4). Error bars represent mean ± SD. P values were calculated using t tests. Post hoc Bonferroni’s test was performed in C and D. **P < 0.01, ***P < 0.001.

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