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Citations to this article

PD-L1– and calcitriol-dependent liposomal antigen-specific regulation of systemic inflammatory autoimmune disease
Ryan Galea, … , Brendan J. O’Sullivan, Ranjeny Thomas
Ryan Galea, … , Brendan J. O’Sullivan, Ranjeny Thomas
Published September 5, 2019
Citation Information: JCI Insight. 2019;4(18):e126025. https://doi.org/10.1172/jci.insight.126025.
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Research Article Nephrology Article has an altmetric score of 75

PD-L1– and calcitriol-dependent liposomal antigen-specific regulation of systemic inflammatory autoimmune disease

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Abstract

Autoimmune diseases resulting from MHC class II–restricted autoantigen-specific T cell immunity include the systemic inflammatory autoimmune conditions rheumatoid arthritis and vasculitis. While currently treated with broad-acting immunosuppressive drugs, a preferable strategy is to regulate antigen-specific effector T cells (Teffs) to restore tolerance by exploiting DC antigen presentation. We targeted draining lymph node (dLN) phagocytic DCs using liposomes encapsulating 1α,25-dihydroxyvitamin D3 (calcitriol) and antigenic peptide to elucidate mechanisms of tolerance used by DCs and responding T cells under resting and immunized conditions. PD-L1 expression was upregulated in dLNs of immunized relative to naive mice. Subcutaneous administration of liposomes encapsulating OVA323–339 and calcitriol targeted dLN PD-L1hi DCs of immunized mice and reduced their MHC class II expression. OVA323–339/calcitriol liposomes suppressed expansion, differentiation, and function of Teffs and induced Foxp3+ and IL-10+ peripheral Tregs in an antigen-specific manner, which was dependent on PD-L1. Peptide/calcitriol liposomes modulated CD40 expression by human DCs and promoted Treg induction in vitro. Liposomes encapsulating calcitriol and disease-associated peptides suppressed the severity of rheumatoid arthritis and Goodpasture’s vasculitis models with suppression of antigen-specific memory T cell differentiation and function. Accordingly, peptide/calcitriol liposomes leverage DC PD-L1 for antigen-specific T cell regulation and induce antigen-specific tolerance in inflammatory autoimmune diseases.

Authors

Ryan Galea, Hendrik J. Nel, Meghna Talekar, Xiao Liu, Joshua D. Ooi, Megan Huynh, Sara Hadjigol, Kate J. Robson, Yi Tian Ting, Suzanne Cole, Karyn Cochlin, Shannon Hitchcock, Bijun Zeng, Suman Yekollu, Martine Boks, Natalie Goh, Helen Roberts, Jamie Rossjohn, Hugh H. Reid, Ben J. Boyd, Ravi Malaviya, David J. Shealy, Daniel G. Baker, Loui Madakamutil, A. Richard Kitching, Brendan J. O’Sullivan, Ranjeny Thomas

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 Total
Citations: 2 4 7 6 9 4 32
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2020 (4)

Title and authors Publication Year
Regulatory T Cells Induced by Single-Peptide Liposome Immunotherapy Suppress Islet-Specific T Cell Responses to Multiple Antigens and Protect from Autoimmune Diabetes
AS Bergot, I Buckle, S Cikaluru, JL Naranjo, CM Wright, G Zheng, M Talekar, EE Hamilton-Williams, R Thomas
Journal of immunology (Baltimore, Md. : 1950) 2020
Calcitriol inhibits osteoclastogenesis in an inflammatory environment by changing the proportion and function of T helper cell subsets (Th2/Th17)
CS Bi, X Li, HL Qu, LJ Sun, Y An, YL Hong, BM Tian, FM Chen
Cell Proliferation 2020
The solid progress of nanomedicine
JP Martins, J das Neves, M de la Fuente, C Celia, H Florindo, N Günday-Türeli, A Popat, JL Santos, F Sousa, R Schmid, J Wolfram, B Sarmento, HA Santos
Drug Delivery and Translational Research 2020
Antigen-Specific Immunotherapy for Treatment of Autoimmune Liver Diseases
N Richardson, ST Ng, DC Wraith
Frontiers in immunology 2020

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