(A) Individual plots represent mean ± SEM of relative abundance determined from summed ion current intensity values for each oxidative enzyme, representing AA and SS microparticles (n = 3) at 0- and 30-hour incubations (37°C); measurements were determined by peptide MS1 chromatogram intensities that were combined for each protein into protein-level data. (B and C) Volcano plots (0 and 30 hours) represent average relative fold differences determined by plotting P values (–log₁₀) for each protein against the calculated fold change (log₂) difference (of that protein) in SS MP relative to AA MP peptide MS1 chromatogram intensities that were combined for each protein into protein-level data. ub@ and phosphoserine@ refer to the posttranslational modifications ubiquitination and serine phosphorylation (at the specified amino acid position), respectively. All peptide-spectrum matches were obtained using a strict 1% protein FDR. P values were generated by applying a t test statistic to log₂-transformed fold change values for each mouse biological condition. All points (for both volcano plots) on or above the horizontal line parallel to the x axis represent proteins with P values of 0.05 or lower. All points on or to the left of the first vertical line parallel to the y axis represent proteins that were downregulated in SS mice 1.5-fold or greater. All points on or to the right of the second vertical line parallel to the y axis represent proteins that were upregulated in SS mice 1.5-fold or greater. Bars represent average mean value; each dot in the bars represents an individual data point, and vertical error bars represent SEM. Student’s t test, 2-tailed, *P < 0.05 vs. corresponding AA 0-hour samples.