Diet-induced obesity was induced in Wistar rats by feeding a high-fat (HF) diet for 12 weeks. A control group was fed a standard chow (SC) diet for the same time period. After the feeding period, rats were then subjected to dietary intervention periods of 2 or 8 weeks as described in Figure 1C. (A) Epididymal white adipose tissue (EWAT) weights for the groups described in Figure 1C, fed the indicated diets for 2 or 8 weeks (2W, 8W, respectively). ⁺P < 0.05 compared with HF group after 2 weeks of diet intervention. ^@P < 0.05 when compared with HF and HF/FG groups after 2 weeks of diet intervention. ^#P < 0.05 when compared with the other 3 groups after 8 weeks of diet intervention. (B) Liver weights for the groups described in Figure 1C, fed the indicated diets for 2 or 8 weeks (2W, 8W, respectively). ⁺P < 0.05 when compared with HF group after 2 weeks of diet intervention. *P < 0.05 when compared with HF group after 8 weeks of diet intervention. In A and B, tissue weights were normalized to body weight. n = 6 for each group. Data are presented as mean ± SD. (C) Glucose infusion rate during hyperinsulinemic-euglycemic clamps. A cohort of animals treated as described in Figure 1C were used. Data are presented as mean ± SD for n = 5–7 rats per group. ⁺P < 0.05 when compared with other groups after 2 weeks of diet intervention. *P < 0.05 when compared with other groups after 8 weeks of diet interventions. For A–C, 2-tailed, unpaired t tests were performed. P < 0.05 with a Bonferroni correction was used to define statistical significance among groups.