3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs
Ranu Surolia, Fu Jun Li, Zheng Wang, Huashi Li, Gang Liu, Yong Zhou, Tracy Luckhardt, Sejong Bae, Rui-ming Liu, Sunad Rangarajan, Joao de Andrade, Victor J. Thannickal, and Veena B. Antony
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Published April 20, 2017 - More info
JCI Insight. 2017;2(8):e94088. https://doi.org/10.1172/jci.insight.94088.
Copyright © 2017, American Society for Clinical Investigation
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Abstract
Idiopathic pulmonary fibrosis (IPF) is a fatal progressive fibrotic lung disease characterized by the presence of invasive myofibroblasts in the lung. Currently, there are only two FDA-approved drugs (pirfenidone and nintedanib) for the treatment of IPF. There are no defined criteria to guide specific drug therapy. New methodologies are needed not only to predict personalized drug therapy, but also to screen novel molecules that are on the horizon for treatment of IPF. We have developed a model system that exploits the invasive phenotype of IPF lung tissue. This ex vivo 3D model uses lung tissue from patients to develop pulmospheres. Pulmospheres are 3D spheroids composed of cells derived exclusively from primary lung biopsies and inclusive of lung cell types reflective of those in situ, in the patient. We tested the pulmospheres of 20 subjects with IPF and 9 control subjects to evaluate the responsiveness of individual patients to antifibrotic drugs. Clinical parameters and outcomes were also followed in the same patients. Our results suggest that pulmospheres simulate the microenvironment in the lung and serve as a personalized and predictive model for assessing responsiveness to antifibrotic drugs in patients with IPF.
Authors
Ranu Surolia, Fu Jun Li, Zheng Wang, Huashi Li, Gang Liu, Yong Zhou, Tracy Luckhardt, Sejong Bae, Rui-ming Liu, Sunad Rangarajan, Joao de Andrade, Victor J. Thannickal, Veena B. Antony
Original citation: JCI Insight. 2017;2(2):e91377. https://doi.org/10.1172/jci.insight.91377.
Citation for this corrigendum: JCI Insight. 2017;2(8):e94088. https://doi.org/10.1172/jci.insight.94088.
The legend for Figure 1B incorrectly identified the subjects from whom the depicted pulmospheres originated. The correct sentence is below.
(B) Immunofluorescent staining of pulmospheres from control patients for α-SMA, SPC, CD31, CD11b, fibronectin-EDA, collagen type I, and collagen type IV in paraffin-embedded sections.
The authors regret the error.
