(A) Percentages of CD11b⁺Ly6C^hiCCR2⁺ monocytes, CD11b⁺Ly6C^intLy6G⁺ neutrophils, and CD11b⁺Ly6C^– cells in the peritonea of BALB/c.Ncf1** and WT mice before and 14 days after injection of pristane. Scatter plots show individual values, means, and SEM (n = 5–8). ***P < 0.001 as determined by ANOVA with Bonferroni post-hoc test. (B) Representative fluorescence microscopy images and (C) quantification of peritoneal cells isolated 1 day after injection of pristane and stained for DNA with propidium iodide (PI, red) and for citrullinated histone H3 (citH3, green). Scale bars: 100 μm. Scatter plot shows individual values, means, and SEM (n = 4–8). PI⁺citH3⁺ events represent cells having undergone NETosis. ***P < 0.001 as determined by ANOVA with Bonferroni post-hoc test. (D) Concentrations of selected cytokines and chemokines in peritoneal lavages of BALB/c.Ncf1** and WT mice 0 and 14 days after injection of pristane. Scatter plot shows individual values, means, and SEM (n = 10). **P < 0.01, ***P < 0.001 as determined by ANOVA with Bonferroni post-hoc test. (E) Quantitative analysis of NETing cells in blood drawn from untreated BALB/c WT and Ncf1** mice. Scatter plot shows individual values, mean, and SEM of percentages of cells having undergone NET formation (PI⁺citH3⁺ events) in 7 to 8 mice. *P < 0.05 as determined by 2-tailed Student’s t test. (F) Percentage of CD11b⁺Ly6C^int Ly6G⁺ cells in the peripheral blood mononuclear cell fraction after density gradient centrifugation performed on blood of BALB/c WT and Ncf1** mice. Scatter plot shows individual values, mean, and SEM, n = 9. *P < 0.05 as determined by 2-tailed Student’s t test using Welch’s correction for unequal variances. LDG, low-density granulocyte. (G) Spontaneous and antimycin A–induced (AMA-induced) mitochondrial ROS production (MitoSOX mean fluorescence intensity [MFI]) in BALB/c WT and Ncf1** mice. Scatter plots show individual values, means, and SEM of 4 to 5 animals. n.s., not significant.