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Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge
Yekyung Seong, … , Harry B. Greenberg, Eugene C. Butcher
Yekyung Seong, … , Harry B. Greenberg, Eugene C. Butcher
Published March 23, 2017
Citation Information: JCI Insight. 2017;2(6):e90233. https://doi.org/10.1172/jci.insight.90233.
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Research Article Immunology Vascular biology

Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge

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Abstract

Antibody-secreting cells are generated in regional lymphoid tissues and traffic as plasmablasts (PBs) via lymph and blood to target sites for local immunity. We used multiparameter flow cytometry to define PB trafficking programs (TPs, combinations of adhesion molecules and chemoattractant receptors) and their imprinting in patients in response to localized infection or immune insults. TPs enriched after infection or autoimmune inflammation of mucosae correlate with sites of immune response or symptoms, with different TPs imprinted during small intestinal, colon, throat, and upper respiratory immune challenge. PBs induced after intramuscular or intradermal influenza vaccination, including flu-specific antibody–secreting cells, display TPs characterized by the lack of mucosal homing receptors. PBs of healthy donors display diverse mucosa-associated TPs, consistent with homeostatic immune activity. Identification of TP signatures of PBs may facilitate noninvasive monitoring of organ-specific immune responses.

Authors

Yekyung Seong, Nicole H. Lazarus, Lusijah Sutherland, Aida Habtezion, Tzvia Abramson, Xiao-Song He, Harry B. Greenberg, Eugene C. Butcher

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Figure 1

Plasmablast frequency in donor blood.

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Plasmablast frequency in donor blood.
(A) Percentage of plasmablasts (PB...
(A) Percentage of plasmablasts (PBs) (log scale) among total lymphocytes. Black symbols represent patients with PB frequencies ≥ 0.25%, dark gray symbols are samples with PBs below 0.25%. (B) IgA+ and IgA– (predominantly IgG) PB percentages. The statistical significance of IgA+ versus IgA– frequencies within a patient class is indicated (paired ratio, 2-tailed t test). For each patient class, the total number of samples (and those with PB frequencies ≥ 0.25% in parentheses) are as follows: gastrointestinal infection (GI), 13 (8); ulcerative colitis (UC), 19 (5); celiac disease (CeD), 7 (2); upper respiratory infection (URI), 25 (13); streptococcal infection (Strep), 6 (4); intramuscular trivalent influenza vaccination (TIV), 28 (7); intradermal TIV (ID-TIV), 13 (5); healthy donor (HD), 14 (0).

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