Biliary atresia is an obstructive cholangiopathy of infancy that progresses to end-stage cirrhosis. Although the pathogenesis of the disease is not completely understood, previous reports link TNFα to apoptosis of the bile duct epithelium in the presence of IFNγ. Here, we investigate if TNFα signaling regulates pathogenic mechanisms of biliary atresia. First, we quantified the expression of
Pranavkumar Shivakumar, Tatsuki Mizuochi, Reena Mourya, Sridevi Gutta, Li Yang, Zhenhua Luo, Jorge A. Bezerra
Activation markers and cytokine/chemokine expression following blockade of TNFα or TNFR1/2.
Flow cytometric quantification of Nkg2d and CD69 in naive NK cells after coculture with RRV-primed DC in the presence of antibodies against TNFR1 and/or TNFR2 (