(A) Upper panel: Western blots of integrin α5, integrin β1, p53, and actin expression in the various fallopian tube nonciliated epithelial (FNE) cell lines. Three independent experiments were performed. Lower panel: Representative video clips (n = 5/condition) of FNE-m-p53^R175H or DF30 cell clusters treated with the indicated function-blocking Abs and cultured in serum/EGF-free medium in suspension for 24 hours (Supplemental Video 9, A and B). Arrows point to dying cells. Five movies were recorded per cell type with 100–150 cells per movie. Scale bar: 150 μm. (B) Representative (n = 15) pseudocolored fluorescence images of ethidium bromide (EtBr) incorporation into GFP-labeled FNE-m-p53^R175H cell clusters treated with the indicated function-blocking Abs and cultured in serum/EGF-free medium for 48 hours. Scale bar: 100 μm. (C) Quantification of EtBr incorporation into FNE-mp53^R175H cell clusters cultured in suspension in serum/EGF-free media in the presence of the indicated function-blocking Abs at the indicated concentrations. This experiment was repeated 3 times at the 2.7 μg/ml concentration of integrin function–blocking Abs and at the 4 μg/ml of mucin 16 (MUC16)– or N-cadherin–blocking Abs, with n = 5 cell clusters scored per condition. Each cell cluster consisted of 100–150 cells. (D) Representative (n = 15) pseudocolored fluorescence images of EtBr incorporation into GFP-labeled DF30 cell clusters treated with the indicated concentration of function-blocking Abs and cultured in serum/EGF-free medium in suspension for 48 hours. Scale bar: 100 μm. (E) Quantification of EtBr incorporation into DF30 cell clusters treated with function-blocking Abs at the indicated concentrations and cultured in suspension in serum/EGF-free media for 48 hours. This experiment was repeated 3 times at the 1.4 μg/ml concentration of integrin-blocking Abs and at the 4 μg/ml of mucin 16 (MUC16)– or N-cadherin–blocking Abs with n = 5 cell clusters analyzed per condition. Each cell cluster consisted of 100–150 cells. All data shown as the median (horizontal bar), interquartile range (box), and minimum/maximum values (whiskers). *P < 0.05 for each condition relative to the samples treated with MUC16 Ab determined by a 1-way ANOVA and post-hoc Tukey-Kramer test comparing each condition to the MUC16 control. NS, not significant difference relative to the MUC16 control.