The gastrointestinal (GI) tract of wild-type (WT) and Disheveled 1 knockout (Dvl1^–/–) mice were processed for histology, RT-PCR, and in situ hybridization. (A) Sections from the ileum of 12-week-old mice were stained with DAPI (nuclei) and immunostained for E-cadherin and lysozyme, and representative images are shown. (B) Sections from the ileum of 12-week-old mice were stained with DAPI and immunostained for MMP7, and representative images are shown. Scale bar: 100 μm. (C) Quantification of the number of DAPI⁺lysozyme⁺ cells per crypt are presented as box plot (Bonferroni *P < 0.004 for comparing the results of the Dvl1^–/– with those of the WT mice). (D) Sections from the GI tract of 8-week-old mice were processed for in situ hybridization and probed for Defa5 and representative images are shown. Scale bar: 200 μm. (E) Sections from the in vitro gut organoids were stained with DAPI and immunostained for lysozyme and EphB2, and representative images are shown. Scale bar: 100 μm. (F) Quantification of the numbers of DAPI⁺lysozyme⁺EphB2⁺ cells per crypt are presented as box plot (Bonferroni *P < 0.003 for comparing the results of the Dvl1^–/– with those of the WT mice). (G) RNA from ileum and proximal colon (PC) of 12-week-old WT and Dvl1^–/– mice was extracted and RT-PCR was performed. The levels of Atoh1/Math1, Lyz1, Defa5, Defa1, EphB2, and EphB3 were normalized to Gapdh and are presented as box plot (n = 5, Bonferroni *P < 0.05, **P < 0.005 for comparing the results of the Dvl1^–/– with those of the WT mice).