Usage Information
Citation Information: JCI Insight. 2024;9(24):e182700. https://doi.org/10.1172/jci.insight.182700.
Abstract
Macrophages are required for healthy repair of the lungs following injury, but they are also implicated in driving dysregulated repair with fibrosis. How these 2 distinct outcomes of lung injury are mediated by different macrophage subsets is unknown. To assess this, single-cell RNA-Seq was performed on lung macrophages isolated from mice treated with LPS or bleomycin. Macrophages were categorized based on anatomic location (airspace versus interstitium), developmental origin (embryonic versus recruited monocyte derived), time after inflammatory challenge, and injury model. Analysis of the integrated dataset revealed that macrophage subset clustering was driven by macrophage origin and tissue compartment rather than injury model. Gpnmb-expressing recruited macrophages that were enriched for genes typically associated with fibrosis were present in both injury models. Analogous GPNMB-expressing macrophages were identified in datasets from both fibrotic and nonfibrotic lung disease in humans. We conclude that this subset represents a conserved response to tissue injury and is not sufficient to drive fibrosis. Beyond this conserved response, we identified that recruited macrophages failed to gain resident-like programming during fibrotic repair. Overall, fibrotic versus nonfibrotic tissue repair is dictated by dynamic shifts in macrophage subset programming and persistence of recruited macrophages.
Authors
Emily M. King, Yifan Zhao, Camille M. Moore, Benjamin Steinhart, Kelsey C. Anderson, Brian Vestal, Peter K. Moore, Shannon A. McManus, Christopher M. Evans, Kara J. Mould, Elizabeth F. Redente, Alexandra L. McCubbrey, William J. Janssen
Usage data is cumulative from November 2024 through December 2024.
| Usage | JCI | PMC |
|---|---|---|
| Text version | 1,102 | 0 |
| 361 | 0 | |
| Figure | 91 | 0 |
| Supplemental data | 328 | 0 |
| Citation downloads | 33 | 0 |
| Totals | 1,915 | 0 |
| Total Views | 1,915 | |
Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.
Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.
