Abstract
Graves’ disease (GD) is an autoimmune condition that can progress to Graves’ ophthalmopathy (GO), leading to irreversible damage to orbital tissues and potential blindness. The pathogenic mechanism is not fully understood. In this study, we conducted single-cell multi-omics analyses on healthy individuals, patients with GD without GO, newly diagnosed patients with GO, and treated patients with GO. Our findings revealed gradual systemic inflammation during GO progression, marked by overactivation of cytotoxic effector T cell subsets, and expansion of specific T cell receptor clones. Importantly, we observed a decline in the immunosuppressive function of activated Treg (aTreg) accompanied by a cytotoxic phenotypic transition. In vitro experiments revealed that dysfunction and transition of GO-autoreactive Treg were regulated by the yin yang 1 (YY1) upon secondary stimulation of thyroid stimulating hormone receptor (TSHR) under inflammatory conditions. Furthermore, adoptive transfer experiments of the GO mouse model confirmed infiltration of these cytotoxic Treg into the orbital lesion tissues. Notably, these cells were found to upregulate inflammation and promote pathogenic fibrosis of orbital fibroblasts (OFs). Our results reveal the dynamic changes in immune landscape during GO progression and provide direct insights into the instability and phenotypic transition of Treg, offering potential targets for therapeutic intervention and prevention of autoimmune diseases.
Authors
Zhong Liu, Shu-Rui Ke, Zhuo-Xing Shi, Ming Zhou, Li Sun, Qi-Hang Sun, Bing Xiao, Dong-Liang Wang, Yan-Jin Huang, Jin-Shan Lin, Hui-Shi Wang, Qi-Kai Zhang, Cai-Neng Pan, Xuan-Wei Liang, Rong-Xin Chen, Zhen Mao, Xian-Chai Lin
Figure 2
The dynamic transcriptional characteristics of T cell subpopulations during the progression of GO.
