(A and B) Representative IF staining images and quantification (right) of (A) IL1B (red) and (B) TNFA (green) in injured tendon sections treated with either rmNGF or GW groups after tenotomy. Black arrows indicate positive cells. Scale bar: 5 μm. n = 5 biological replicates. (C) Representative TB staining images and quantification (right) of the mast cells in injured tendon sections treated with either rmNGF or GW groups after tenotomy. The total number of mast cells was counted. Black arrows indicate mast cells. Scale bar: 5 μm. n = 5 biological replicates. (D) Representative IF staining images and quantification (right) of CAM1 (green) in injured tendon sections treated with either rmNGF or GW groups after tenotomy. Yellow arrows indicate positive cells. Scale bar: 5 μm. n = 5 biological replicates. (E) Representative μCT 3D modeling images of Achilles tendons (sagittal view) in indicated group after tenotomy and quantification (right) of ectopic BV. The red rectangular dashed box represents the reconstruction image of the ectopic bone. Scale bar: 2 mm. n = at least 6 biological replicates. (F) Representative IF staining images and quantification (right) of CAM1 (green) in Achilles tendon sections of C57BL/6J and Kit^W-sh/W-sh mice treated with or without rmNGF groups after tenotomy, with DAPI counterstaining (blue). The number of CAM1⁺ cells was counted. The white dashed line indicates the margin of the Achilles tendon and calcaneus. Yellow arrows indicate activated mast cells. Scale bar: 20 μm. n = 5 biological replicates. All data are representative of 2 independent experiments. Data were shown as mean ± SD and compared with 1-way ANOVA with Tukey’s multiple-comparison test.