(A) Volcano plot of the proteomics data showing reduced expression of B7-H3 and SDCBP (red dots) in U87-MG cells treated with shRNA targeting AURKA. (B and C) The protein expression levels of B7-H3, total AURKA, SDCBP, p-EGFR (Y1068), and total EGFR in LN18 cells (B) and U87-MG cells (C) expressing shNC or AURKA-shRNA#3 were detected via Western blotting, and the quantifications are shown on the right. β-Actin was used as the internal control. (D) B7-H3 expression on the cell surface in U87-MG cells expressing shNC or AURKA-shRNA#3 was detected by flow cytometry, and the quantification of the results is shown on the right. Cells that were only stained with isotype control antibodies were used as the negative control (NC). (E) The protein expression levels of B7-H3, total AURKA, SDCBP, p-EGFR (Y1068), and total EGFR in LN229 cells expressing vector or AURKA_cDNA_Flag were detected via Western blotting, and the quantifications are shown on the right. β-Actin was used as the internal control. (F) B7-H3 expression on the cell surface in LN229 cells expressing vector or AURKA_cDNA_Flag was detected by flow cytometry, and the quantification of the results is shown on the right. Cells that were only stained with isotype control antibodies were used as the negative control (NC). All samples were biologically independent, and 3 independent experiments were performed. Comparisons were performed via 1-tailed unpaired Student’s t test (A) or 2-tailed unpaired Student’s t test (B–F). The data are presented as the mean ± SD (B–F). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.