(A) Heatmap representing log₂FC in transcription of 18 inflammasome genes on each sorted myeloid cell subset from the peripheral blood (PB) from n = 4 RA versus n = 4 healthy controls (HC) (red, upregulated; blue, downregulated). Size of yellow dots is proportional to statistical significance between PB RA and healthy donors (FDR-corrected P < 0.05). (B) Venn diagram showing overlap of DEG associated with the inflammasome by IPA in the indicated myeloid cell subsets from the PB of n = 4 patients with RA compared with n = 4 healthy donors. (C) Gene network including significantly upregulated (red) or downregulated (blue) DEG inflammasome genes in PB CD1c⁺ cDC from patients with RA compared with HC (right). Individual (purple) and connected target genes (red) are shown. (D) mRNA expression of NLRC4 (left plot) and NLRP3 (right plot) relative to β-actin validated by qPCR in sorted CD1c⁺ cDC from n = 5 patients with RA and n = 4 HC individuals. Statistical significance was calculated with a 2-tailed Mann Whitney U test. *P < 0.05. (E) Unsupervised heatmap reflecting normalized expression levels of the indicated inflammasome sensors in Mo, CD1c⁺ cDC, and CD141⁺ cDC from n = 3 SF of patients with RA versus the same myeloid subsets from the blood of n = 4 HC. (F–H) Fold change on IL-1β and CCL3 mRNA expression relative to β-actin mRNA levels analyzed by qPCR (F), on surface expression of CD64 (G), and on functional ability to induce pathogenic IFN-γ⁺IL-17⁺ cells (H) in CD1c⁺ cDC nucleofected with indicated siRNAs and cultured in media or in the presence of IgG-dsDNA complexes (n = 7 experiments). Statistical significance was calculated using a 2-tailed Wilcoxon matched-pairs test. *P < 0.05.