Go to The Journal of Clinical Investigation
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Immunology
    • Metabolism
    • Nephrology
    • Oncology
    • Pulmonology
    • All ...
  • Videos
  • Collections
    • Resource and Technical Advances
    • Clinical Medicine
    • Reviews
    • Editorials
    • Perspectives
    • Top read articles
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • In-Press Preview
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Transfers
  • Advertising
  • Job board
  • Contact
Immunologic timeline of Ebola virus disease and recovery in humans
Anita K. McElroy, … , Stuart T. Nichol, Christina F. Spiropoulou
Anita K. McElroy, … , Stuart T. Nichol, Christina F. Spiropoulou
Published May 21, 2020
Citation Information: JCI Insight. 2020;5(10):e137260. https://doi.org/10.1172/jci.insight.137260.
View: Text | PDF
Research Article Immunology Virology

Immunologic timeline of Ebola virus disease and recovery in humans

  • Text
  • PDF
Abstract

A complete understanding of human immune responses to Ebola virus infection is limited by the availability of specimens and the requirement for biosafety level 4 (BSL-4) containment. In an effort to bridge this gap, we evaluated cryopreserved PBMCs from 4 patients who survived Ebola virus disease (EVD) using an established mass cytometry antibody panel to characterize various cell populations during both the acute and convalescent phases. Acute loss of nonclassical monocytes and myeloid DCs, especially CD1c+ DCs, was noted. Classical monocyte proliferation and CD38 upregulation on plasmacytoid DCs coincided with declining viral load. Unsupervised analysis of cell abundance demonstrated acute declines in monocytic, NK, and T cell populations, but some populations, many of myeloid origin, increased in abundance during the acute phase, suggesting emergency hematopoiesis. Despite cell losses during the acute phase, upregulation of Ki-67 correlated with recovery of cell populations over time. These data provide insights into the human immune response during EVD.

Authors

Anita K. McElroy, Rama S. Akondy, David R. Mcllwain, Han Chen, Zach Bjornson-Hooper, Nilanjan Mukherjee, Aneesh K. Mehta, Garry Nolan, Stuart T. Nichol, Christina F. Spiropoulou

×

Figure 2

Frequencies and activation status of various cell populations.

Options: View larger image (or click on image) Download as PowerPoint
Frequencies and activation status of various cell populations.
(A) Frequ...
(A) Frequencies of T cells, B cells, basophils, and NK cells as a percentage of CD45+CD66– leukocytes in patients compared with normal healthy controls (range for all controls in gray). Fold increase in median Ki-67 signal as compared with that in a control population is shown for NK and NKT cells at each patient time point. D2, day 2. (B) The expanded Lin–CD14–CD16–HLA-DR– but CD11b+ population that was noted in EVD9 is shown, with histograms demonstrating pSTAT3 and Ki-67 expression in these cells.

Copyright © 2022 American Society for Clinical Investigation
ISSN 2379-3708

Sign up for email alerts