(A) The picture (left) and the plot for body weights (right) at P14. Statistical significance was determined by 1-way ANOVA. Mean ± SEM of 4–10 experimental animals. ***P < 0.001. (B) The brains at P14. (C) IF analysis for CIC in the cerebral cortex. Scale bar: 100 μm. (D) H&E staining of Cic^WT and Cic^KO brains. Scale bar: 1 mm. For B–D, 3 brains per group were examined, and representative images are shown. (E) Quantification of the thickness cerebral cortex (left) and cerebellar cortex (right) are plotted. Mean ± SEM of 3 experimental animals. (F) GSEA in Cic^KO versus Cic^WT brains. Two brains per group were analyzed. (G and H) IF analysis for NeuN, SATB2, and CTIP2 in the cerebral cortex of P14 mouse. Scale bar: 100 μm. (I) Quantifications at layers 2–4 of NeuN⁺ or SATB2⁺ and layers 5–6 of CTIP2⁺ numbers are plotted. Mean ± SEM of 200 DAPI⁺ nuclei from 3 animals. (J) Measurement the thickness for SATB2⁺ layers 2–4 and CTIP2⁺ layers 5–6. Mean ± SEM of 3 images from 3 animals. (K) WB analysis for indicated protein expressions in cerebral cortex lysates. Samples were run on 3 gels, and the most representative ACTB blot is shown. (L) Densitometry analysis of multiple WB in J is plotted. Mean ± SEM of 3 experimental animals. (M) qPCR results of indicated genes in P14 Cic^KO versus Cic^WT brains. Mean ± SEM of 5–7 experimental animals. For I, J, L, and M, statistical significance was determined by unpaired t test. ***P < 0.001.