The most severe manifestation of peripheral arterial disease (PAD) is critical limb ischemia (CLI). CLI patients suffer high rates of amputation and mortality; accordingly, there remains a clear need both to better understand CLI and to develop more effective treatments. Gastrocnemius muscle was obtained from 32 older (51–84 years) non-PAD controls, 27 claudicating PAD patients (ankle-brachial index [ABI] 0.65 ± 0.21 SD), and 19 CLI patients (ABI 0.35 ± 0.30 SD) for whole transcriptome sequencing and comprehensive mitochondrial phenotyping. Comparable permeabilized myofiber mitochondrial function was paralleled by both similar mitochondrial content and related mRNA expression profiles in non-PAD control and claudicating patient tissues. Tissues from CLI patients, despite being histologically intact and harboring equivalent mitochondrial content, presented a unique bioenergetic signature. This signature was defined by deficits in permeabilized myofiber mitochondrial function and a unique pattern of both nuclear and mitochondrial encoded gene suppression. Moreover, isolated muscle progenitor cells retained both mitochondrial functional deficits and gene suppression observed in the tissue. These findings indicate that muscle tissues from claudicating patients and non-PAD controls were similar in both their bioenergetics profile and mitochondrial phenotypes. In contrast, CLI patient limb skeletal muscles harbor a unique skeletal muscle mitochondriopathy that represents a potentially novel therapeutic site for intervention.
Terence E. Ryan, Dean J. Yamaguchi, Cameron A. Schmidt, Tonya N. Zeczycki, Saame Raza Shaikh, Patricia Brophy, Thomas D. Green, Michael D. Tarpey, Reema Karnekar, Emma J. Goldberg, Genevieve C. Sparagna, Maria J. Torres, Brian H. Annex, P. Darrell Neufer, Espen E. Spangenburg, Joseph M. McClung
Title and authors | Publication | Year |
---|---|---|
Mesenchymal Stem Cell Transplantation for Ischemic Diseases: Mechanisms and Challenges
TT Nguyen, NB Vu, PV Pham |
Tissue Engineering and Regenerative Medicine | 2021 |
Intrinsic OXPHOS limitations underlie cellular bioenergetics in leukemia
MA Nelson, KL McLaughlin, JT Hagen, HS Coalson, C Schmidt, M Kassai, KA Kew, JM McClung, PD Neufer, P Brophy, NA Vohra, D Liles, MC Cabot, KH Fisher-Wellman |
eLife | 2021 |
Racial differences in the limb skeletal muscle transcriptional programs of patients with critical limb ischemia
ZS Terwilliger, TE Ryan, EJ Goldberg, CA Schmidt, DJ Yamaguchi, R Karnekar, P Brophy, TD Green, TN Zeczycki, FM Gabhann, BH Annex, JM McClung |
Vascular medicine (London, England) | 2021 |
Unique Metabolomic Profile of Skeletal Muscle in Chronic Limb Threatening Ischemia
RB Khattri, K Kim, T Thome, ZR Salyers, KA OMalley, SA Berceli, ST Scali, TE Ryan |
Journal of Clinical Medicine | 2021 |
The Role of Mitochondrial Function in Peripheral Arterial Disease: Insights from Translational Studies
A Gratl, S Wipper, JP Frese, B Raude, A Greiner, D Pesta |
International journal of molecular sciences | 2021 |
Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
E Granier, MO Zakari, MB Alsahly, LG Koch, S Britton, LC Katwa, RM Lust |
Frontiers in Cardiovascular Medicine | 2021 |