Abstract
Authors
Xiao Yang, Sameen Fatima, Salvador Sampere-Birlanga, Akshay Ware, Mariana Shumliakivska, Lukas Zanders, Srisurekha Radhakrishnan, Guillermo Luxán, David John, Stefan Günther, Silvia Mas-Peiro, Stefanie Dimmeler, Andreas M. Zeiher, Wesley T. Abplanalp
Figure 1
TET2-mutant carriers and cells exhibit enhanced phagocytic signatures in aged individuals.
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(A ) UMAP of PBMCs from aged individuals with or without TET2 -CHIP, showing major immune populations. (B ) Phagocytosis module scores across immune cell types. (C ) Phagocytosis module scores in monocytes from No-CHIP controls and TET2 -mutant individuals. (D ) Mutation-resolved single-cell RNA-seq identifying WT and TET2 -mutant cells. (E ) Phagocytosis module scores in WT versus TET2 -mutant monocytes. (F and G ) Representative flow cytometry plots and quantification of β-amyloid uptake in siTET2 and control monocytes. (H ) Fluorescence microscopy of β-amyloid uptake in monocytes. Statistics: Wilcoxon rank-sum test (C and E ); paired 2-tailed Student’s t test (G ). Data are means ± SEM. *P < 0.05.
