(A) Experimental approach. (B) Flow cytometry analyses of peripheral blood. Each dot represents a mouse (n = 11–24). (C) Tumor burden and grade at 16 weeks. Each dot represents a mouse (n = 30–32). (D) Images of lymphoid aggregates (LAs) in non-tumor bearing lungs. (E) IHC of lung from Tet2 KO mouse (top) and relative quantification within LAs. Each color represents data from a single mouse (n = 4–11). (F) IHC quantification of immune cells within tumors. Each color represents data from a single mouse (n = 9–11). Data are from 2 or 3 independent experiments at 16-week time point (color-coded in B and C). Circles represent color-coded means, and data are shown as mean ± SEM. Statistical analyses were performed with 1-way ANOVA tests (B, Tukey, E, Brown-Forsythe and Welch nested), in C with the Mann-Whitney U test, and in F with 2-tailed nested t test of nested. T, tumor. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.