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High protein does not change autophagy in human PBMCs after 1 hour
Sanjna Singh, … , Julien Bensalem, Timothy J. Sargeant
Sanjna Singh, … , Julien Bensalem, Timothy J. Sargeant
Published July 15, 2025
Citation Information: JCI Insight. 2025;10(16):e188845. https://doi.org/10.1172/jci.insight.188845.
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Research Letter Cell biology Metabolism

High protein does not change autophagy in human PBMCs after 1 hour

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Abstract

Authors

Sanjna Singh, Célia Fourrier, Kathryn J. Hattersley, Leanne K. Hein, Jemima Gore, Alexis Martin, Linh V.P. Dang, Barbara King, Rachael A. Protzman, Paul J. Trim, Leonie K. Heilbronn, Julien Bensalem, Timothy J. Sargeant

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Figure 1

A high-protein meal does not alter autophagic flux in PBMCs.

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A high-protein meal does not alter autophagic flux in PBMCs.
(A) Study d...
(A) Study design. (B and C) Fasted and postprandial plasma glucose (paired 2-tailed t test) and insulin (Wilcoxon’s test), n = 41; (D) branched-chain amino acids (paired 2-tailed t test), n = 15; (E) PBMC S6 phosphorylation (Wilcoxon’s test), n = 15; (F) autophagic flux (Wilcoxon’s test), n = 40; (G) Spearman’s correlations between blood measurements and baseline characteristics; and (H) 2-way ANOVA of autophagic flux.

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