CD154:CD11b blockade enhances CD8+ T cell differentiation during infection but not transplantation
Katie L. Alexander, Kelsey B. Bennion, Danya Liu, Mandy L. Ford
Katie L. Alexander, Kelsey B. Bennion, Danya Liu, Mandy L. Ford
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Research Article Immunology Transplantation

CD154:CD11b blockade enhances CD8+ T cell differentiation during infection but not transplantation

Abstract

CD154 is a promising target for immunosuppression in transplantation, autoimmunity, and inflammatory diseases. We previously identified CD11b as a novel alternative receptor for CD154 during alloimmunity. However, the impact of specific CD154:CD11b blockade on immune responses to infection has not been well characterized. Here, we have shown that in contrast with its immunosuppressive effect on graft-specific CD8+ T cells, CD154:CD11b blockade unexpectedly improved both the quantity and quality of murine herpesvirus-68–specific CD8+ T cells as measured by an increase in tetramer-positive KLRG1loCD127hi memory precursor effector cells. The differential effect of CD154:CD11b blockade on graft- versus virus-specific CD8+ T cells was underpinned by differences in phosphorylated S6 downstream of mTOR complex 1; however, differential expression of key transcription factors Eomes and TCF-1 was dictated by the type of antigen stimulus. These data demonstrate that priming conditions play an important role in determining the outcome of immunotherapy and suggest that specific inhibition of CD154:CD11b interactions could be effective for suppressing alloimmune responses while maintaining protective immunity to minimize infectious complications following transplantation.

Authors

Katie L. Alexander, Kelsey B. Bennion, Danya Liu, Mandy L. Ford

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Figure 1

CD154:CD11b blockade alone attenuates the magnitude of the CD8+ T cell response to an allograft.

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CD154:CD11b blockade alone attenuates the magnitude of the CD8+ T cell r...
(A) CD45.1+ congenic mice received 1 × 106 BALB/c-specific TCR-transgenic 2C splenocytes and BALB/c skin grafts, as well as either no further treatment or cM7 on days 0, 2, 4, and 6. Skin grafts, draining lymph nodes (DLNs), spleen, and blood were collected 10 days posttransplantation. (B) Representative flow cytometry plots and summary data of frequencies of Thy1.1+ 2C cells of CD3+CD8+ T cells in the spleen. (C) Representative flow cytometry plots and summary data of frequencies of infiltrating CD3+CD8+ T cells of H-2Kb+ cells in skin allografts. (D) Representative flow cytometry plots and summary data of frequencies of IL-2– and IFN-γ–producing cells of CD44+CD8+ T cells following restimulation with PMA and ionomycin. Data are representative of 3 individual experiments with 5–14 mice/group. *P < 0.05, **P < 0.01 by Mann-Whitney test or 2-way ANOVA with Šidák’s test where appropriate.