(A) Experimental scheme of BMDM isolation and treatment from mice stimulated with β-glucan. BMDM were treated with BMP inhibitor; after 24 hours, cells were stimulated with LPS for 24 hours. (B) BMDM Id1 gene expression. (C) BMDM Il-6 gene expression. (D) BMDM Tnf gene expression. (E) Experimental scheme for mouse model of bone metastasis with β-glucan stimulus and BMP inhibition (n = 5). Seven days prior to seeding a syngeneic bone metastasis cell line in the tibia, mice were stimulated with β-glucan. Twenty-eight–day osmotic pumps were implanted into mice 7 days after intratibial injection. (F) Intratibial tumor volume. (G) Peripheral blood PyMT gene expression. (H) Lung macrometastases. (I) Experimental scheme for mouse model of bone metastasis with β-glucan stimulus and BMP inhibition (n = 5). Seven days prior to seeding a bone metastasis cell line in the tibia, mice were stimulated with β-glucan, and 7-day osmotic pumps were implanted into mice. Intratibial injection of a syngeneic bone metastasis cell line was performed after 7 days. (J) Intratibial tumor volume. (K) Peripheral blood PyMT gene expression. (L) Lung macrometastases. (M) Experimental scheme for mouse model of bone metastasis with β-glucan stimulus in LysMCre CTL (n = 7) and LysMCre BMPR1a-cKO (n = 4) mice. Seven days prior to seeding a bone metastasis cell line in the tibia, mice were stimulated with β-glucan. (N) Kaplan-Meier curve of overall survival from intratibial bone metastasis seeding to mouse sacrifice or death. (O) Lung macrometastases. Data are presented as mean ± SEM. Statistical values determined by ordinary 1-way ANOVA followed by post hoc analysis for Tukey multiple comparisons (B–D). Statistical values determined by Student’s t test (F–H, J–L, and O). Statistical value determined by log-rank test (N).