Abstract
Idiopathic pulmonary fibrosis (IPF) is an age-related interstitial lung disease, characterized by inadequate alveolar regeneration and ectopic bronchiolization. While some molecular pathways regulating lung progenitor cells have been described, the role of metabolic pathways in alveolar regeneration is poorly understood. We report that expression of fatty acid oxidation (FAO) genes is significantly diminished in alveolar epithelial cells of IPF lungs by single-cell RNA sequencing and tissue staining. Genetic and pharmacological inhibition in AT2 cells of carnitine palmitoyltransferase 1a (CPT1a), the rate-limiting enzyme of FAO, promoted mitochondrial dysfunction and acquisition of aberrant intermediate states expressing basaloid, and airway secretory cell markers SCGB1A1 and SCGB3A2. Furthermore, mice with deficiency of CPT1a in AT2 cells show enhanced susceptibility to developing lung fibrosis with an accumulation of epithelial cells expressing markers of intermediate cells, airway secretory cells, and senescence. We found that deficiency of CPT1a causes a decrease in SMAD7 protein levels and TGF-β signaling pathway activation. These findings suggest that the mitochondrial FAO metabolic pathway contributes to the regulation of lung progenitor cell repair responses and deficiency of FAO contributes to aberrant lung repair and the development of lung fibrosis.
Authors
Quetzalli D. Angeles-Lopez, Jhonny Rodriguez-Lopez, Paula Agudelo Garcia, Jazmin Calyeca, Diana Álvarez, Marta Bueno, Lan N. Tu, Myriam Salazar-Terreros, Natalia Vanegas-Avendaño, Jordan E. Krull, Aigul Moldobaeva, Srimathi Bogamuwa, Stephanie S. Scott, Victor Peters, Brenda F. Reader, Sruti Shiva, Michael Jurczak, Mahboobe Ghaedi, Qin Ma, Toren Finkel, Mauricio Rojas, Ana L. Mora
Figure 2
Inhibition of CPT1a in AT2 cells induces the emergence of RAS and basaloid cell phenotypes in human organoids.
