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Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2
Danielle R. Adney, … , Stephanie N. Seifert, Vincent J. Munster
Danielle R. Adney, … , Stephanie N. Seifert, Vincent J. Munster
Published November 22, 2022
Citation Information: JCI Insight. 2022;7(22):e159573. https://doi.org/10.1172/jci.insight.159573.
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Research Article COVID-19

Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2

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Abstract

An animal model that fully recapitulates severe COVID-19 presentation in humans has been a top priority since the discovery of SARS-CoV-2 in 2019. Although multiple animal models are available for mild to moderate clinical disease, models that develop severe disease are still needed. Mink experimentally infected with SARS-CoV-2 developed severe acute respiratory disease, as evident by clinical respiratory disease, radiological, and histological changes. Virus was detected in nasal, oral, rectal, and fur swabs. Deep sequencing of SARS-CoV-2 from oral swabs and lung tissue samples showed repeated enrichment for a mutation in the gene encoding nonstructural protein 6 in open reading frame 1ab. Together, these data indicate that American mink develop clinical features characteristic of severe COVID-19 and, as such, are uniquely suited to test viral countermeasures.

Authors

Danielle R. Adney, Jamie Lovaglio, Jonathan E. Schulz, Claude Kwe Yinda, Victoria A. Avanzato, Elaine Haddock, Julia R. Port, Myndi G. Holbrook, Patrick W. Hanley, Greg Saturday, Dana Scott, Carl Shaia, Andrew M. Nelson, Jessica R. Spengler, Cassandra Tansey, Caitlin M. Cossaboom, Natalie M. Wendling, Craig Martens, John Easley, Seng Wai Yap, Stephanie N. Seifert, Vincent J. Munster

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Figure 4

Pulmonary histopathology and IHC.

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Pulmonary histopathology and IHC.
(A) Lung to BW ratio, Mann-Whitney tes...
(A) Lung to BW ratio, Mann-Whitney test. Graph depicts median with individuals. (B) Diffusely consolidated dark-mottled red lungs. (C) Multifocal pulmonary congestion (box). (D) Enlarged section of image C, vascular thrombi (arrows) and alveolar edema (asterisks). (E) Alveolar fibrin (arrows). (F) Lymphoplasmacytic perivascular cuffing (arrows). (G) Pulmonary PTAH staining (black). (H) Enlarged section of G, microthrombi (arrows). Scale bar expressed in μm in lower right corner of each image.

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